ArticleBMC microbiology2008
Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Enhancing Probiotic Performance in Food Matrices: From Strain Selection to Bioengineering.Journal of food science · 2026Review
- In Situ Cross-Linked Porous Starch Microencapsulation Enhances the Colonization ofFoods (Basel, Switzerland) · 2025Article
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Biotechnological Applications of Probiotics: A Multifarious Weapon to Disease and Metabolic Abnormality.Probiotics and antimicrobial proteins · 2022Review
- Probiotic engineering strategies for the heterologous production of antimicrobial peptides.Advanced drug delivery reviews · 2021Review
- Rehydration before Application Improves Functional Properties of LyophilizedMicroorganisms · 2021Article
- Role of probiotics in prevention and treatment of enteric infections: a comprehensive review.3 Biotech · 2021Review
- Recent Development of ProbioticFrontiers in bioengineering and biotechnology · 2021Review
- Enhancing water stress tolerance improves fitness in biological control strains of Lactobacillus plantarum in plant environments.PloS one · 2018Article
- Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices.Frontiers in microbiology · 2018Article
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- Harnessing bacteriocin biology as targeted therapy in the GI tract.Gut microbes · 2016Article
- Advances in the Microbiome: Applications to Clostridium difficile Infection.Journal of clinical medicine · 2016Review
- Designer probiotics: Development and applications in gastrointestinal health.World journal of gastrointestinal pathophysiology · 2015Review
- Under the microscope: From pathogens to probiotics and back.Bioengineered · 2015Review
- Metagenomics and novel gene discovery: promise and potential for novel therapeutics.Virulence · 2014Review
- Bifidobacteria-host interactions--an update on colonisation factors.BioMed research international · 2014Review
- Cathelicidin protects against Helicobacter pylori colonization and the associated gastritis in mice.Gene therapy · 2013Article
- Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome.The ISME journal · 2012Article
- A bile-inducible membrane protein mediates bifidobacterial bile resistance.Microbial biotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000.
resultsIn vitro bile tolerance of both strains was significantly enhanced (P < 0.001), following heterologous expression of the Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P < 0.001), than control strains from the faeces and intestines of mice (n = 5), following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice.
conclusionCollectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational genetic manipulation and that this can significantly improve delivery to and colonisation of the GI tract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.