ArticleProceedings of the National Academy of Sciences of the United States of America2008
Integrin alpha2beta1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 220 citations in OpenAlex.
- Enhanced Efficacy of Sandblasted, Large-Grit, Alkaline-Etched (SLA) Titanium Surfaces Combined with Plasma Treatment and Secretory Leukocyte Protease Inhibitor (SLPI) Coating in Promoting Osteoblast Activity and Reducing Bacterial Adhesion.Journal of functional biomaterials · 2026Article
- Polystyrene substrate effects on human dental pulp cell behavior: implications for in vitro culture optimization.BMC oral health · 2025Article
- Bone Marrow Stromal Cells Generate a Pro-Healing Inflammasome When Cultured on Titanium-Aluminum-Vanadium Surfaces with Microscale/Nanoscale Structural Features.Biomimetics (Basel, Switzerland) · 2025Article
- Beyond microroughness: novel approaches to navigate osteoblast activity on implant surfaces.International journal of implant dentistry · 2024Review
- Wnt16 Increases Bone-to-Implant Contact in an Osteopenic Rat Model by Increasing Proliferation and Regulating the Differentiation of Bone Marrow Stromal Cells.Annals of biomedical engineering · 2024Article
- Osteoporosis GWAS-implicatedJBMR plus · 2024Article
- An overview of 3D printed metal implants in orthopedic applications: Present and future perspectives.Heliyon · 2023Review
- Bone marrow stromal cells generate an osteoinductive microenvironment when cultured on titanium-aluminum-vanadium substrates with biomimetic multiscale surface roughness.Biomedical materials (Bristol, England) · 2023Article
- How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate.International journal of molecular sciences · 2023Review
- Analysis of disordered abrasive scratches on titanium surfaces and their impact on nuclear translocation of yes-associated protein.Scientific reports · 2022Article
- Topography-mediated immunomodulation in osseointegration; Ally or Enemy.Biomaterials · 2022Review
- Canonical Wnt signaling enhances pro-inflammatory response to titanium by macrophages.Biomaterials · 2022Article
- Tailoring of TiAl6V4 Surface Nanostructure for Enhanced In Vitro Osteoblast Response via Gas/Solid (Non-Line-of-Sight) Oxidation/Reduction Reactions.Biomimetics (Basel, Switzerland) · 2022Article
- Extracellular Vesicles of Mesenchymal Stromal Cells Can be Taken Up by Microglial Cells and Partially Prevent the Stimulation Induced by β-amyloid.Stem cell reviews and reports · 2022Article
- Evolution of Bioactive Implants in Lateral Interbody Fusion.International journal of spine surgery · 2022Article
- Integrins in the Regulation of Mesenchymal Stem Cell Differentiation by Mechanical Signals.Stem cell reviews and reports · 2022Review
- Implant-bone-interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo.Bioengineering & translational medicine · 2022Review
- Can activated titanium interbody cages accelerate or enhance spinal fusion? a review of the literature and a design for clinical trials.Journal of materials science. Materials in medicine · 2021Review
- E-cigarette Aerosol Mixtures Inhibit Biomaterial-Induced Osseointegrative Cell Phenotypes.Materialia · 2021Article
- Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium.Journal of biomedical materials research. Part A · 2021Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 4 countries.
Funding
Abstract
Efforts to improve bone response to biomaterials have focused on ligands that bind alpha5beta1 integrins. However, antibodies to alpha5beta1 reduce osteoblast proliferation but do not affect differentiation when cells are grown on titanium (Ti). beta1-silencing blocks the differentiation stimulus of Ti microtopography, suggesting that other beta1 partners are important. Stably alpha2-silenced MG63 human osteoblast-like cells were used to test whether alpha2beta1 specifically mediates osteoblast response to Ti surface micron-scale structure and energy. WT and alpha2-silenced MG63 cells were cultured on tissue culture polystyrene (TCPS) and Ti disks with different surface microtopographies: machined pretreatment (PT) surfaces [mean peak to valley roughness (R(a)) < 0.02 microm], PT surfaces that were grit-blasted and acid-etched (SLA; R(a) = 4 microm), and SLA with high surface energy (modSLA). Alkaline phosphatase (ALP), alpha2 and beta1 mRNA, but not alpha5, alpha v, beta3, type-I collagen, or osteocalcin, increased on SLA and modSLA at 6 days. Alpha2 increased at 8 days on TCPS and PT, but remained unchanged on SLA and modSLA. Alpha2-protein was reduced 70% in alpha2-siRNA cells, whereas alpha5-mRNA and protein were unaffected. Alpha2-knockdown blocked surface-dependent increases in beta1 and osteocalcin and decreases in cell number and increases in ALP and local factors typical of MG63 cells grown on SLA and modSLA [e.g., prostaglandin E(2), osteoprotegerin, latent and active TGF-beta1, and stimulatory effects of 1alpha,25(OH)(2)D(3) on these parameters]. This finding indicates that alpha2beta1 signaling is required for osteoblastic differentiation caused by Ti microstructure and surface energy, suggesting that conclusions based on cell behavior on TCPS are not predictive of behavior on other substrates or the mechanisms involved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.