ArticleMolecular biology of the cell2008
SNARE function is not involved in early endosome docking.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes.Cell reports · 2023Article
- SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.Nature communications · 2019Article
- Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.Molecular biology of the cell · 2018Article
- Probing and manipulating intracellular membrane traffic by microinjection of artificial vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- SRpHi ratiometric pH biosensors for super-resolution microscopy.Nature communications · 2017Article
- Calcium sensitive ring-like oligomers formed by synaptotagmin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- Multiple and distinct strategies of yeast SNAREs to confer the specificity of membrane fusion.Scientific reports · 2014Article
- Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell-free assay.Traffic (Copenhagen, Denmark) · 2014Article
- Pannexin 1 ohnologs in the teleost lineage.The Journal of membrane biology · 2012Article
- Cell-free fusion of bacteria-containing phagosomes with endocytic compartments.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- An update on transport vesicle tethering.Molecular membrane biology · 2010Review
- A fluorescence-based in vitro assay for investigating early endosome dynamics.Nature protocols · 2010Article
- Organelle docking: R-SNAREs are late.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Minimal membrane docking requirements revealed by reconstitution of Rab GTPase-dependent membrane fusion from purified components.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Sorting in early endosomes reveals connections to docking- and fusion-associated factors.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Docking and fusion of transport vesicles constitute elementary steps in intracellular membrane traffic. While docking is thought to be initiated by Rab-effector complexes, fusion is mediated by SNARE (N-ethylmaleimide-sensitive factor [NSF] attachment receptor) proteins. However, it has been recently debated whether SNAREs also play a role in the establishment or maintenance of a stably docked state. To address this question, we have investigated the SNARE dependence of docking and fusion of early endosomes, one of the central sorting compartments in the endocytic pathway. A new, fluorescence-based in vitro assay was developed, which allowed us to investigate fusion and docking in parallel. Similar to homotypic fusion, docking of early endosomes is dependent on the presence of ATP and requires physiological temperatures. Unlike fusion, docking is insensitive to the perturbation of SNARE function by means of soluble SNARE motifs, SNARE-specific F(ab) fragments, or by a block of NSF activity. In contrast, as expected, docking is strongly reduced by interfering with the synthesis of phosphatidyl inositol (PI)-3 phosphate, with the function of Rab-GTPases, as well as with early endosomal autoantigen 1 (EEA1), an essential tethering factor. We conclude that docking of early endosomes is independent of SNARE function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.