ArticleJournal of clinical microbiology2008
Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
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- Article
- Serotyping ofFood safety (Tokyo, Japan) · 2018Article
- Distributions of Salmonella subtypes differ between two U.S. produce-growing regions.Applied and environmental microbiology · 2014Article
- Comparison of typing methods with a new procedure based on sequence characterization for Salmonella serovar prediction.Journal of clinical microbiology · 2013Article
- Several enteropathogens are circulating in suckling and newly weaned piglets suffering from diarrhea in the province of Villa Clara, Cuba.Tropical animal health and production · 2013Article
- Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica.FEMS microbiology letters · 2012Article
- Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives.Applied and environmental microbiology · 2011Review
- Microbial Diagnostic Microarrays for the Detection and Typing of Food- and Water-Borne (Bacterial) Pathogens.Microarrays (Basel, Switzerland) · 2011Review
- Article
- Evaluation of a DNA microarray, the check-points ESBL/KPC array, for rapid detection of TEM, SHV, and CTX-M extended-spectrum beta-lactamases and KPC carbapenemases.Antimicrobial agents and chemotherapy · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The commercial PremiTest Salmonella kit uses a multiplexed DNA typing test aimed at identifying common serovars of Salmonella enterica. It was used in assays over a 9-month period in the Belgian reference laboratory that performs the routine identification of Salmonella strains of animal origin. A blind analysis of 754 strains was conducted in parallel by classical serotyping and the PremiTest assay. Full results were available for 685 strains (90.8%) by serotyping, while the remaining 69 strains were found to be nontypeable due to either a lack of surface antigen expression or autoagglutination properties. When the PremiTest assay (version 4.2) was performed with crude bacterial extracts, it identified 658 strains (87.3%), including most strains found to be nontypeable by serotyping. In contrast, it gave no, wrong, dual, or noninterpretable results for 96 strains, for which 23 were caused by assay failures. When purified DNA instead of crude extracts were tested, the number of strains successfully identified to the serovar level increased to 714 (94.7%), while all assay failures were cleared. Our conclusion is that, in its actual development stage, the application of the investigated kit to purified DNA samples offers a valuable alternative to classical serotyping for laboratories performing the routine identification of Salmonella strains belonging to commonly encountered serovars and isolated from a given geographical area, assuming that the system has been validated beforehand with a significant number of strains originating from that particular area.
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