ArticleMalaria journal2008
Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures.
Article in Malaria journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Chemo-proteomics in antimalarial target identification and engagement.Medicinal research reviews · 2023Review
- Cultivation of Asexual Intraerythrocytic Stages ofPathogens (Basel, Switzerland) · 2023Review
- Long-TermThe American journal of tropical medicine and hygiene · 2019Article
- Thermodynamic concepts in the study of microbial populations: age structure in Plasmodium falciparum infected red blood cells.PloS one · 2011Article
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8 authors.
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Abstract
backgroundIn vitro cultivation of Plasmodium falciparum is usually carried out through the continuous preservation of infected erythrocytes deposited in static thin layers of settled haematocrit. This technique, called the candle-jar method, was first achieved by Trager and Jensen in 1976 and has undergone slight modifications since then. However, no systematic studies concerning the geometry of the haematocrit layer have been carried out. In this work, a thorough investigation of the effects of the geometric culturing conditions on the parasite's development is presented.
methodsSeveral experimental trials exploring different settings have been carried out, covering haematocrit layer depths that ranged from 6 mm to 3 mm and separation between the walls of the culturing device that ranged from 7.5 mm to 9 mm. The obtained results have been analysed and compared to different system-level models and to an Individual-Based Model.
conclusionIn line with the results, a mechanism governing the propagation of the infection which limits it to the vicinity of the interface between the haematocrit layer and the culture medium is deduced, and the most appropriate configurations are proposed for further experimental assays.
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