ArticleAnalytical chemistry2008
Micropatterned fluid lipid bilayer arrays created using a continuous flow microspotter.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- Distinct Binding Properties of Neutravidin and Streptavidin Proteins to Biotinylated Supported Lipid Bilayers: Implications for Sensor Functionalization.Sensors (Basel, Switzerland) · 2022Article
- Supported lipid bilayer platforms to probe cell mechanobiology.Biochimica et biophysica acta. Biomembranes · 2017Review
- Multiplexed Lipid Bilayers on Silica Microspheres for Analytical Screening Applications.Analytical chemistry · 2017Article
- Hierarchical hydrodynamic flow confinement: efficient use and retrieval of chemicals for microscale chemistry on surfaces.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Latest developments in experimental and computational approaches to characterize protein-lipid interactions.Proteomics · 2012Review
- High-throughput screening of drug-lipid membrane interactions via counter-propagating second harmonic generation imaging.Analytical chemistry · 2011Article
- Using micropatterned lipid bilayer arrays to measure the effect of membrane composition on merocyanine 540 binding.Biochimica et biophysica acta · 2011Article
- Characterization of new serum biomarkers in breast cancer using lipid microarrays.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2010Article
- Stable, ligand-doped, poly(bis-SorbPC) lipid bilayer arrays for protein binding and detection.ACS applied materials & interfaces · 2009Article
- Analysis of oxidative stress biomarkers using a simultaneous competitive/non-competitive micromosaic immunoassay.Analytica chimica acta · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a new method for creating micropatterned lipid bilayer arrays (MLBAs) using a 3D microfluidic system. An array of fluid lipid membranes was patterned onto a glass substrate using a Continuous Flow Microspotter. Fluorescence microscopy experiments were used to verify the formation of a bilayer structure on the glass substrate. Fluorescence recovery after photobleaching experiments demonstrated the bilayers' fluidity was maintained while being individually corralled on the substrate. The reproducibility of bilayer formation within an array was demonstrated by the linear response of membrane fluorescence versus mol % rhodamine functionalized lipids incorporated into the vesicles prior to fusion to the surface. The highly customizable nature of the MLBAs was demonstrated utilizing three different fluorescently labeled lipids to generate a multiple component lipid array. Finally, the cholera toxin B/ganglioside GM 1, antidinitrophenyl (DNP) antibody/DNP, and NeutrAvidin/biotin protein-ligand systems were used to model multiple protein-ligand binding on the MLBAs. The multicomponent patterned bilayers were functionalized with GM 1, DNP, and biotin lipids, and binding curves was generated by recording surface fluorescence versus increasing concentration of membrane bound ligands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.