ArticleLangmuir : the ACS journal of surfaces and colloids2008
DNA release dynamics from reducible polyplexes by atomic force microscopy.
Article in Langmuir : the ACS journal of surfaces and colloids, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Polycondensed Peptide-Based Polymers for Targeted Delivery of Anti-Angiogenic siRNA to Treat Endometriosis.International journal of molecular sciences · 2023Article
- A review of the tortuous path of nonviral gene delivery and recent progress.International journal of biological macromolecules · 2021Review
- Effect of Double Substitution in Cationic Chitosan Derivatives on DNA Transfection Efficiency.Polymers · 2020Article
- Stimuli-Responsive Gold Nanoparticles for Cancer Diagnosis and Therapy.Journal of functional biomaterials · 2016Review
- Rapid Exchange Between Free and Bound States in RNA-Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release.Biomacromolecules · 2016Article
- Endosomolytic reducible polymeric electrolytes for cytosolic protein delivery.Biomacromolecules · 2013Article
- Intrinsic dynamics of DNA-polymer complexes: a mechanism for DNA release.Molecular pharmaceutics · 2012Article
- Reducible DNA nanoparticles enhance in vitro gene transfer via an extracellular mechanism.Journal of controlled release : official journal of the Controlled Release Society · 2010Article
- DNA release dynamics from bioreducible layer-by-layer films.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Multifunctional Dendrimer-templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection.Advanced functional materials · 2010Article
- Effect of innate glutathione levels on activity of redox-responsive gene delivery vectors.Journal of controlled release : official journal of the Controlled Release Society · 2010Article
- DNA release dynamics from bioreducible poly(amido amine) polyplexes.The journal of physical chemistry. B · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Controlled intracellular disassembly of polyelectrolyte complexes of polycations and DNA (polyplexes) is a crucial step for the success of nonviral gene delivery. Motivated by our previous observation of different gene delivery performances among multiblock reducible copolypeptide vectors ( Manickam, D. S. ; Oupicky, D. Bioconjugate Chem. 2006, 17, 1395- 1403 ), atomic force microscopy is used to visualize plasmid DNA in various decondensed states from reducible polypeptide polyplexes under simulated physiological reducing conditions. DNA decondensation is triggered by reductive degradation of disulfide-containing cationic polypeptides. Striking differences in DNA release dynamics between polyplexes based on polypeptides of histidine-rich peptide (HRP, CKHHHKHHHKC) and nuclear localization signal (NLS, CGAGPKKKRKVC) peptide are presented. The HRP and NLS polyplexes are similar to each other in their initial morphology with a majority of them containing only one DNA plasmid. Upon reductive degradation by dithiothreitol, DNA is released from NLS abruptly regardless of the initial polyplex morphology, while DNA release from HRP polyplexes displays a gradual decondensation that is dependent on the size of polyplexes. The release rate is higher for larger HRP polyplexes. The smaller HRP polyplexes become unstable when they are in contact with expanding chains nearby. The results reveal potentially rich DNA release dynamics that can be controlled by subtle variation in multivalent counterion binding to DNA as well as the cellular matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.