ArticleCurrent microbiology2008
Long-term survival of Legionella pneumophila in the viable but nonculturable state after monochloramine treatment.
Article in Current microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Review
- Article
- Comparison between Legiolert and real time PCR in the detection of Legionella pneumophila from environmental water samples.PloS one · 2025Article
- Intracellular ATP concentration is a key regulator of bacterial cell fate.Journal of bacteriology · 2024Article
- Legionella colonization and 3D spatial location within a Pseudomonas biofilm.Scientific reports · 2024Article
- Effects of Copper onPathogens (Basel, Switzerland) · 2024Article
- Kicking sleepers out of bed: Macrophages promote reactivation of dormant Cryptococcus neoformans by extracellular vesicle release and non-lytic exocytosis.PLoS pathogens · 2023Article
- Wake Up! Resuscitation of Viable but Nonculturable Bacteria: Mechanism and Potential Application.Foods (Basel, Switzerland) · 2022Review
- Detection of Diverse Sequence Types of Legionella pneumophila by Legiolert Enzymatic-Based Assay and the Development of a Long-Term Storage Protocol.Microbiology spectrum · 2022Article
- Tenets of a holistic approach to drinking water-associated pathogen research, management, and communication.Water research · 2022Review
- Risk assessment and quantitative measurement along with monitoring ofNew microbes and new infections · 2022Article
- Article
- Legionella and other opportunistic pathogens in full-scale chloraminated municipal drinking water distribution systems.Water research · 2021Article
- Review
- Comparison of two culture methods for the enumeration of Legionella pneumophila from potable water samples.Journal of water and health · 2021Article
- Assessment of hygienic conditions of recreational facility restrooms: an integrated approach.Journal of preventive medicine and hygiene · 2021Article
- Induction and Resuscitation of the Viable but Non-culturable (VBNC) State inFrontiers in microbiology · 2019Article
- Peptidyl-Prolyl-Infection and immunity · 2019Article
- Persistent presence of outer membrane epitopes during short- and long-term starvation of five Legionella pneumophila strains.BMC microbiology · 2018Article
- Diagnostic testing for Legionnaires' disease.Annals of clinical microbiology and antimicrobials · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila, a facultative intracellular human pathogen, can persist for long periods in natural and artificial aquatic environments. Eradication of this bacterium from plumbing systems is often difficult. We tested L. pneumophila survival after monochloramine treatment. Survival was monitored using the BacLight Bacterial Viability Kit (Molecular Probes), ChemChrome V6 Kit (Chemunex), quantitative polymerase chain reaction and culturability on buffered charcoal-yeast extract agar. In nonculturable samples, regain of culturability was obtained after addition of the amoeba Acanthamoeba castellanii, and esterase activity and membrane integrity were observed after >4 months after treatment. These results demonstrate for the first time that L. pneumophila could persist for long periods in biofilms into the viable but nonculturable (VBNC) state. Monitoring L. pneumophila in water networks is generally done by enumeration on standard solid medium. This method does not take into account VBNC bacteria. VBNC L. pneumophila could persist for long periods and should be resuscitated by amoeba. These cells constitute potential sources of contamination and should be taken into account in monitoring water networks.
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Registered trials
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