ArticleCurrent microbiology2009
Characterization of a rolling-circle replication plasmid pLR1 from Lactobacillus plantarum LR1.
Article in Current microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 11 citations in OpenAlex.
- Characterization and sequencing analysis of pLP2.5-11 and pLP3.0-4 novel cryptic plasmids from3 Biotech · 2023Article
- Complete genome sequencing of3 Biotech · 2019Article
- Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1-3.Current microbiology · 2016Article
- Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments.International journal of molecular sciences · 2015Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cryptic plasmid from Lactobacillus plantarum LR1, designated pLR1, was sequenced and characterized. It consisted of a 2066-bp circular molecule with a G + C content of 52.7%. The plasmid pLR1 was predicted to contain five putative ORFs, in which ORF1 shared 93% and 92% identity with Rep proteins of pLP1 and pC30il, members of rolling-circle replication (RCR) pC194 family. Detection of single-stranded DNA (ssDNA) intermediates by Southern hybridization and mung bean nuclease treatment confirmed that pLR1 replicated via the RCR mechanism. Accumulation of ssDNA in rifampicin-treated strains implied that the host-coded RNA polymerase was involved in the conversion of ssDNA to double-stranded DNA (dsDNA). Furthermore, the copy number of pLR1 was estimated to be 36 in each cell by real-time polymerase chain reaction.
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