ArticleProceedings of the National Academy of Sciences of the United States of America2008
Rapid Virulence Annotation (RVA): identification of virulence factors using a bacterial genome library and multiple invertebrate hosts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria.Nature chemistry · 2022Article
- The differential strain virulence of the candidate toxins of3 Biotech · 2020Article
- Review
- A predation assay using amoebae to screen for virulence factors unearthed the first W. chondrophila inclusion membrane protein.Scientific reports · 2019Article
- TheeLife · 2019Article
- Transcriptional up-regulation of the TGF-β intracellular signaling transducer Mad of Drosophila larvae in response to parasitic nematode infection.Innate immunity · 2018Article
- Coping with Environmental Eukaryotes; Identification ofMicroorganisms · 2018Article
- Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity.PLoS pathogens · 2017Article
- Understanding pine wilt disease: roles of the pine endophytic bacteria and of the bacteria carried by the disease-causing pinewood nematode.MicrobiologyOpen · 2017Review
- Virulence factor activity relationships (VFARs): a bioinformatics perspective.Environmental science. Processes & impacts · 2017Review
- Article
- From Insect to Man: Photorhabdus Sheds Light on the Emergence of Human Pathogenicity.PloS one · 2015Article
- An insecticidal compound produced by an insect-pathogenic bacterium suppresses host defenses through phenoloxidase inhibition.Molecules (Basel, Switzerland) · 2014Article
- An unbiased method for clustering bacterial effectors using host cellular phenotypes.Applied and environmental microbiology · 2014Article
- Merging chemical ecology with bacterial genome mining for secondary metabolite discovery.Journal of industrial microbiology & biotechnology · 2014Review
- Activated and inactivated immune responses in Caenorhabditis elegans against Photorhabdus luminescens TT01.SpringerPlus · 2014Article
- Ail and PagC-related proteins in the entomopathogenic bacteria of Photorhabdus genus.PloS one · 2014Article
- Article
- Mammalian cell entry genes in Streptomyces may provide clues to the evolution of bacterial virulence.Scientific reports · 2013Article
- Production of cytotoxic glidobactins/luminmycins by Photorhabdus asymbiotica in liquid media and live crickets.Journal of natural products · 2012Article
Corrections and comments
- Erratum issuedYang, Guowei [added]
Authors and funding
12 authors.
Funding
Abstract
Current sequence databases now contain numerous whole genome sequences of pathogenic bacteria. However, many of the predicted genes lack any functional annotation. We describe an assumption-free approach, Rapid Virulence Annotation (RVA), for the high-throughput parallel screening of genomic libraries against four different taxa: insects, nematodes, amoeba, and mammalian macrophages. These hosts represent different aspects of both the vertebrate and invertebrate immune system. Here, we apply RVA to the emerging human pathogen Photorhabdus asymbiotica using "gain of toxicity" assays of recombinant Escherichia coli clones. We describe a wealth of potential virulence loci and attribute biological function to several putative genomic islands, which may then be further characterized using conventional molecular techniques. The application of RVA to other pathogen genomes promises to ascribe biological function to otherwise uncharacterized virulence genes.
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