ArticleNucleic acids research2008
Low conservation and species-specific evolution of alternative splicing in humans and mice: comparative genomics analysis using well-annotated full-length cDNAs.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Identification of Gene Expression and Splicing QTLs in Porcine Muscle Associated with Meat Quality Traits.Animals : an open access journal from MDPI · 2025Article
- Targeting splicing for hematological malignancies therapy.BMC genomics · 2024Review
- Detection of orthologous exons and isoforms using EGIO.Bioinformatics (Oxford, England) · 2022Article
- Overview of PAX gene family: analysis of human tissue-specific variant expression and involvement in human disease.Human genetics · 2021Review
- Aberrant RNA Splicing in Cancer and Drug Resistance.Cancers · 2018Review
- Alternative Splicing in Breast Cancer and the Potential Development of Therapeutic Tools.Genes · 2017Review
- Transcriptome-Wide Analysis Reveals Modulation of Human Macrophage Inflammatory Phenotype Through Alternative Splicing.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Correcting for differential transcript coverage reveals a strong relationship between alternative splicing and organism complexity.Molecular biology and evolution · 2014Article
- Architecture and metamorphosis.Topics in current chemistry · 2014Review
- The relationship between gene isoform multiplicity, number of exons and protein divergence.PloS one · 2013Article
- Origin and evolution of spliceosomal introns.Biology direct · 2012Review
- When orthologs diverge between human and mouse.Briefings in bioinformatics · 2011Review
- Global regulation of alternative splicing during myogenic differentiation.Nucleic acids research · 2010Article
- Assessment of orthologous splicing isoforms in human and mouse orthologous genes.BMC genomics · 2010Article
- Unconstrained mining of transcript data reveals increased alternative splicing complexity in the human transcriptome.Nucleic acids research · 2010Article
- A comprehensive survey of human polymorphisms at conserved splice dinucleotides and its evolutionary relationship with alternative splicing.BMC evolutionary biology · 2010Article
- H-DBAS: human-transcriptome database for alternative splicing: update 2010.Nucleic acids research · 2010Article
- H-InvDB in 2009: extended database and data mining resources for human genes and transcripts.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using full-length cDNA sequences, we compared alternative splicing (AS) in humans and mice. The alignment of the human and mouse genomes showed that 86% of 199 426 total exons in human AS variants were conserved in the mouse genome. Of the 20 392 total human AS variants, however, 59% consisted of all conserved exons. Comparing AS patterns between human and mouse transcripts revealed that only 431 transcripts from 189 loci were perfectly conserved AS variants. To exclude the possibility that the full-length human cDNAs used in the present study, especially those with retained introns, were cloning artefacts or prematurely spliced transcripts, we experimentally validated 34 such cases. Our results indicate that even retained-intron type transcripts are typically expressed in a highly controlled manner and interact with translating ribosomes. We found non-conserved AS exons to be predominantly outside the coding sequences (CDSs). This suggests that non-conserved exons in the CDSs of transcripts cause functional constraint. These findings should enhance our understanding of the relationship between AS and species specificity of human genes.
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