ReviewCytometry. Part A : the journal of the International Society for Analytical Cytology2008
Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.
Review in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- T cell antigenic recognition sequencing (TAR-seq) enables precise characterization of vaccine-elicited cellular immunity ex vivo andFrontiers in immunology · 2026Article
- Antigen-specificity measurements are the key to understanding T cell responses.Frontiers in immunology · 2023Review
- Stitchr: stitching coding TCR nucleotide sequences from V/J/CDR3 information.Nucleic acids research · 2022Article
- Identification and Characterization of Antigen-Specific CD8Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Predicting TCR-Epitope Binding Specificity Using Deep Metric Learning and Multimodal Learning.Genes · 2021Article
- How to dissect the plasticity of antigen-specific immune response: a tissue perspective.Clinical and experimental immunology · 2020Review
- Heat and Chemical Treatments Affect the Viability, Morphology, and Physiology ofApplied and environmental microbiology · 2019Article
- VDJdb: a curated database of T-cell receptor sequences with known antigen specificity.Nucleic acids research · 2018Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies.European journal of immunology · 2017Article
- Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4(+) and CD8(+) memory formation.Immunology · 2015Article
- Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters.Cancer immunology, immunotherapy : CII · 2015Article
- β-cell-specific CD8 T cell phenotype in type 1 diabetes reflects chronic autoantigen exposure.Diabetes · 2015Article
- Multiplex Identification of Antigen-Specific T Cell Receptors Using a Combination of Immune Assays and Immune Receptor Sequencing.PloS one · 2015Article
- Cryopreservation of MHC multimers: Recommendations for quality assurance in detection of antigen specific T cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- MHC-derived allopeptide activates TCR-biased CD8+ Tregs and suppresses organ rejection.The Journal of clinical investigation · 2014Article
- Monitoring of pathogen-specific T-cell immune reconstitution after allogeneic hematopoietic stem cell transplantation.Frontiers in immunology · 2013Review
- Minor histocompatibility antigens and the maternal immune response to the fetus during pregnancy.American journal of reproductive immunology (New York, N.Y. : 1989) · 2013Review
- Allergic airway disease in mice alters T and B cell responses during an acute respiratory poxvirus infection.PloS one · 2013Article
- Missing: a diagnostic technique to enumerate antigen-specific T cells.Critical reviews in oncology/hematology · 2012Review
- Cytometry: today's technology and tomorrow's horizons.Methods (San Diego, Calif.) · 2012Review
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Authors and funding
10 authors.
Funding
Abstract
The ability to quantify and characterize antigen-specific CD8+ T cells irrespective of functional readouts using fluorochrome-conjugated peptide-major histocompatibility complex class I (pMHCI) tetramers in conjunction with flow cytometry has transformed our understanding of cellular immune responses over the past decade. In the case of prevalent CD8+ T cell populations that engage cognate pMHCI tetramers with high avidities, direct ex vivo identification and subsequent data interpretation is relatively straightforward. However, the accurate identification of low frequency antigen-specific CD8+ T cell populations can be complicated, especially in situations where T cell receptor-mediated tetramer binding occurs at low avidities. Here, we highlight a few simple techniques that can be employed to improve the visual resolution, and hence the accurate quantification, of tetramer binding CD8+ T cell populations by flow cytometry. These methodological modifications enhance signal intensity, especially in the case of specific CD8+ T cell populations that bind cognate antigen with low avidities, minimize background noise, and enable improved discrimination of true pMHCI tetramer binding events from nonspecific uptake.
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