ArticleJournal of experimental botany2008
Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins.
Article in Journal of experimental botany, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers, 1 of them a synthesis that pooled it.
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Who cites it
119 citing papers in PubMed, 1 synthesis or guideline pooled it, 230 citations in OpenAlex.
- Grape Berry Secondary Metabolites and Their Modulation by Abiotic Factors in a Climate Change Scenario-A Review.Frontiers in plant science · 2021Pooled it
- Grapevine rootstocks are a promising strategy for phytostabilization in soils contaminated with cadmium.Scientific reports · 2026Article
- Integrated transcriptomic and metabolomic analyses reveal the regulatory network underlying NtGSTU10-mediated nicotine synthesis and transport in tobacco.Scientific reports · 2026Article
- Overexpression of a Tonoplast Malate Transporter Gene Leads to Enhanced Anthocyanin Biosynthesis in Apple.Plant biotechnology journal · 2026Article
- Molecular identification, isolation and functional characterization of a glutathione S-transferase gene CsGST in saffron (Crocus sativus L.).Scientific reports · 2026Article
- Molecular mechanisms of phytohormone ABA-regulated anthocyanin biosynthesis in grape berry: an epigenetic dual-gating hypothesis within a five-layer regulatory framework.Frontiers in molecular biosciences · 2026Review
- A novel function for the transcription factor sensitive to proton rhizotoxicity1 in promoting anthocyanin accumulation in strawberry.Plant biotechnology journal · 2025Article
- Integrated omics and functional insights into BjMYB90-mediated regulation of BjGSTF12 for enhanced anthocyanin biosynthesis in mustard (Brassica juncea).Plant cell reports · 2025Article
- Transcriptome and Metabolome Analyses of Flavonoid Biosynthesis During Berry Development of Muscadine Grape (Plants (Basel, Switzerland) · 2025Article
- Natural variations in the Cis-elements of GhRPRS1 contributing to petal colour diversity in cotton.Plant biotechnology journal · 2024Article
- Haplotype-resolved genome assembly and implementation of VitExpress, an open interactive transcriptomic platform for grapevine.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification and Characterization of the Glutathione S-Transferase Gene Family in Blueberry (Plants (Basel, Switzerland) · 2024Article
- Promoter replication of grape MYB transcription factor is associated with a new red flesh phenotype.Plant cell reports · 2024Article
- Integrated metabolome and transcriptome analyses reveal the role of BoGSTF12 in anthocyanin accumulation in Chinese kale (Brassica oleracea var. alboglabra).BMC plant biology · 2024Article
- Vineyard microclimate alterations induced by black inter-row mulch through transcriptome reshaped the flavoromics of cabernet sauvignon grapes.BMC plant biology · 2024Article
- Non-Mature miRNA-Encoded Micropeptide miPEP166c Stimulates Anthocyanin and Proanthocyanidin Synthesis in Grape Berry Cells.International journal of molecular sciences · 2024Article
- The grapevine ABC transporter B family member 15 is aFrontiers in plant science · 2024Article
- PeGSTU58, a Glutathione S-Transferase fromInternational journal of molecular sciences · 2023Article
- PgMYB1 Positively Regulates Anthocyanin Accumulation by ActivatingInternational journal of molecular sciences · 2023Article
- A glutathione S-transferase GhTT19 determines flower petal pigmentation via regulating anthocyanin accumulation in cotton.Plant biotechnology journal · 2023Article
59 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ligandin activity of specific glutathione S-transferases (GSTs) is necessary for the transport of anthocyanins from the cytosol to the plant vacuole. Five GSTs were purified from Vitis vinifera L. cv. Gamay Fréaux cell suspension cultures by glutathione affinity chromatography. These proteins underwent Edman sequencing and mass spectrometry fingerprinting, with the resultant fragments aligned with predicted GSTs within public databases. The corresponding coding sequences were cloned, with heterologous expression in Escherichia coli used to confirm GST activity. Transcriptional profiling of these candidate GST genes and key anthocyanin biosynthetic pathway genes (PAL, CHS, DFR, and UFGT) in cell suspensions and grape berries against anthocyanin accumulation demonstrated strong positive correlation with two sequences, VvGST1 and VvGST4, respectively. The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize bronze-2 complementation model, providing further evidence for their function as anthocyanin transport proteins in grape cells. Furthermore, the differential induction of VvGST1 and VvGST4 in suspension cells and grape berries suggests functional differences between these two proteins. Further investigation of these candidate ligandins may identify a mechanism for manipulating anthocyanin accumulation in planta and in vitro suspension cells.
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