ArticleApplied and environmental microbiology2008
The Functional quality of soluble recombinant polypeptides produced in Escherichia coli is defined by a wide conformational spectrum.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Immunogenic potential and neutralizing ability of a heterologous version of the most abundant three-finger toxin from the coral snakeThe journal of venomous animals and toxins including tropical diseases · 2024Article
- Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography.Microbial cell factories · 2023Article
- Fluorescence dequenching assay for the activity of TEV protease.Analytical biochemistry · 2022Article
- Article
- Recombinant Protein Production and Purification of Insoluble Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Comparison of Immune Effects BetweenFrontiers in veterinary science · 2020Article
- Fused IgY Fc and Polysaccharide Adjuvant Enhanced the Immune Effect of the Recombinant VP2 and VP5 Subunits-A Prospect for Improvement of Infectious Bursal Disease Virus Subunit Vaccine.Frontiers in microbiology · 2017Article
- Recombinant protein expression in Escherichia coli: advances and challenges.Frontiers in microbiology · 2014Review
- Intracellular CXCR4⁺ cell targeting with T22-empowered protein-only nanoparticles.International journal of nanomedicine · 2012Article
- Recombinant protein quality evaluation: proposal for a minimal information standard.Standards in genomic sciences · 2011Article
- Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli.Microbial cell factories · 2011Article
- Engineering building blocks for self-assembling protein nanoparticles.Microbial cell factories · 2010Article
- Side effects of chaperone gene co-expression in recombinant protein production.Microbial cell factories · 2010Review
- Rehosting of bacterial chaperones for high-quality protein production.Applied and environmental microbiology · 2009Article
- Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm.Microbial cell factories · 2009Article
- Learning about protein solubility from bacterial inclusion bodies.Microbial cell factories · 2009Article
- Cross-system excision of chaperone-mediated proteolysis in chaperone-assisted recombinant protein production.Bioengineered bugsArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.