ArticleApplied and environmental microbiology2008
HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- HtrAs are essential for the survival of the haloarchaeonApplied and environmental microbiology · 2024Article
- Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions.Microbial cell factories · 2018Article
- A review on Lactococcus lactis: from food to factory.Microbial cell factories · 2017Review
- Mechanism of action of the arylomycin antibiotics and effects of signal peptidase I inhibition.Antimicrobial agents and chemotherapy · 2012Article
- Type I signal peptidase and protein secretion in Staphylococcus aureus.Journal of bacteriology · 2012Article
- Article
- Immunization against Leishmania major infection using LACK- and IL-12-expressing Lactococcus lactis induces delay in footpad swelling.PloS one · 2012Article
- Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HtrA is a unique protease on the extracellular surface of Lactococcus lactis. It is known to take part in the proteolysis of many secreted recombinant proteins, and the mutation of htrA can lead to the complete stabilization of recombinant proteins. In this work, we have shown that htrA mutation also leads to significant reduction of the efficiency of recombinant-protein secretion. We also show that the level of HtrA can be lowered by the suppression of the acid tolerance response (ATR) in L. lactis. Instead of using an L. lactis htrA mutant, the reduction of the HtrA level in wild-type recombinant cultures of L. lactis by ATR suppression may serve as a better strategy for the production of secreted recombinant proteins.
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Registered trials
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