ArticlePlant physiology2008
Characterization of cytokinin and adenine transport in Arabidopsis cell cultures.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Cytokinin availability and chloroplast functional lifetime in senescing leaves.Frontiers in plant science · 2026Review
- Spatially distributed cytokinins: Metabolism, signaling, and transport.Plant communications · 2024Review
- Fine Mapping and Candidate Gene Analysis ofInternational journal of molecular sciences · 2022Article
- Differential Subcellular Distribution of Cytokinins: How Does Membrane Transport Fit into the Big Picture?International journal of molecular sciences · 2021Review
- The Hulks and the Deadpools of the Cytokinin Universe: A Dual Strategy for Cytokinin Production, Translocation, and Signal Transduction.Biomolecules · 2021Review
- The Roles of Plant Hormones and Their Interactions with Regulatory Genes in Determining Meristem Activity.International journal of molecular sciences · 2019Review
- Cytokinin Transporters: Multisite Players in Cytokinin Homeostasis and Signal Distribution.Frontiers in plant science · 2019Review
- Modification of Barley Plant Productivity Through Regulation of Cytokinin Content by Reverse-Genetics Approaches.Frontiers in plant science · 2018Article
- Cytokinins on the Move.Frontiers in plant science · 2017Review
- Natural genetic variation in Arabidopsis for responsiveness to plant growth-promoting rhizobacteria.Plant molecular biology · 2016Article
- The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility.Protoplasma · 2016Article
- Cytokinin-Specific Glycosyltransferases Possess Different Roles in Cytokinin Homeostasis Maintenance.Frontiers in plant science · 2016Article
- Cell-Type-Specific Cytokinin Distribution within the Arabidopsis Primary Root Apex.The Plant cell · 2015Article
- Global analysis of the Gossypium hirsutum L. Transcriptome during leaf senescence by RNA-Seq.BMC plant biology · 2015Article
- Cell walls as a stage for intercellular communication regulating shoot meristem development.Frontiers in plant science · 2015Review
- Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP).Journal of experimental botany · 2014Article
- Arabidopsis ABCG14 is essential for the root-to-shoot translocation of cytokinin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Nucleobase and nucleoside transport and integration into plant metabolism.Frontiers in plant science · 2014Review
- Multiscale systems analysis of root growth and development: modeling beyond the network and cellular scales.The Plant cell · 2012Review
- Histidine kinases in plants: cross talk between hormone and stress responses.Plant signaling & behavior · 2012Review
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins are distributed through the vascular system and trigger responses of target cells via receptor-mediated signal transduction. Perception and transduction of the signal can occur at the plasma membrane or in the cytosol. The signal is terminated by the action of extra- or intracellular cytokinin oxidases. While radiotracer studies have been used to study transport and metabolism of cytokinins in plants, little is known about the kinetic properties of cytokinin transport. To provide a reference dataset, radiolabeled trans-zeatin (tZ) was used for uptake studies in Arabidopsis (Arabidopsis thaliana) cell culture. Uptake kinetics of tZ are multiphasic, indicating the presence of both low- and high-affinity transport systems. The protonophore carbonyl cyanide m-chlorophenylhydrazone is an effective inhibitor of cytokinin uptake, consistent with H(+)-mediated uptake. Other physiological cytokinins, such as isopentenyl adenine and benzylaminopurine, are effective competitors of tZ uptake, whereas allantoin has no inhibitory effect. Adenine competes for zeatin uptake, indicating that the degradation product of cytokinin oxidases is transported by the same systems. Comparison of adenine and tZ uptake in Arabidopsis seedlings reveals similar uptake kinetics. Kinetic properties, as well as substrate specificity determined in cell cultures, are compatible with the hypothesis that members of the plant-specific purine permease family play a role in adenine transport for scavenging extracellular adenine and may, in addition, be involved in low-affinity cytokinin uptake.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.