ArticleJournal of bacteriology2008
A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 37 citations in OpenAlex.
- Architecture, Function, Regulation, and Evolution of α-Glucans Metabolic Enzymes in Prokaryotes.Chemical reviews · 2024Review
- Comprehensive genome analysis of3 Biotech · 2023Article
- Salinity stress endurance of the plants with the aid of bacterial genes.Frontiers in genetics · 2023Review
- Bacterial α-diglucoside metabolism: perspectives and potential for biotechnology and biomedicine.Applied microbiology and biotechnology · 2021Review
- The diversity and commonalities of the radiation-resistance mechanisms of Deinococcus and its up-to-date applications.AMB Express · 2019Review
- Article
- Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability.Applied and environmental microbiology · 2019Article
- Radiation resistance in thermophiles: mechanisms and applications.World journal of microbiology & biotechnology · 2017Review
- The Production and Utilization of GDP-glucose in the Biosynthesis of Trehalose 6-Phosphate by Streptomyces venezuelae.The Journal of biological chemistry · 2017Article
- A Highly Stable D-Amino Acid Oxidase of the Thermophilic Bacterium Rubrobacter xylanophilus.Applied and environmental microbiology · 2014Article
- Complete genome sequence of the Radiation-Resistant bacterium Rubrobacter radiotolerans RSPS-4.Standards in genomic sciences · 2014Article
- Correlating microbial diversity patterns with geochemistry in an extreme and heterogeneous environment of mine tailings.Applied and environmental microbiology · 2014Article
- Article
- Cloning, expression and characterization of trehalose-6-phosphate phosphatase from a psychrotrophic bacterium, Arthrobacter strain A3.World journal of microbiology & biotechnology · 2012Article
- Role of trehalose in salinity and temperature tolerance in the model halophilic bacterium Chromohalobacter salexigens.PloS one · 2012Article
- A biosynthetic pathway for BE-7585A, a 2-thiosugar-containing angucycline-type natural product.Journal of the American Chemical Society · 2010Article
- Biochemical characterization of the maltokinase from Mycobacterium bovis BCG.BMC biochemistry · 2010Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Trehalose is the primary organic solute in Rubrobacter xylanophilus under all conditions tested, including those for optimal growth. We detected genes of four different pathways for trehalose synthesis in the genome of this organism, namely, the trehalose-6-phosphate synthase (Tps)/trehalose-6-phosphate phosphatase (Tpp), TreS, TreY/TreZ, and TreT pathways. Moreover, R. xylanophilus is the only known member of the phylum Actinobacteria to harbor TreT. The Tps sequence is typically bacterial, but the Tpp sequence is closely related to eukaryotic counterparts. Both the Tps/Tpp and the TreT pathways were active in vivo, while the TreS and the TreY/TreZ pathways were not active under the growth conditions tested and appear not to contribute to the levels of trehalose observed. The genes from the active pathways were functionally expressed in Escherichia coli, and Tps was found to be highly specific for GDP-glucose, a rare feature among these enzymes. The trehalose-6-phosphate formed was specifically dephosphorylated to trehalose by Tpp. The recombinant TreT synthesized trehalose from different nucleoside diphosphate-glucose donors and glucose, but the activity in R. xylanophilus cell extracts was specific for ADP-glucose. The TreT could also catalyze trehalose hydrolysis in the presence of ADP, but with a very high K(m). Here, we functionally characterize two systems for the synthesis of trehalose in R. xylanophilus, a representative of an ancient lineage of the actinobacteria, and discuss a possible scenario for the exceptional occurrence of treT in this extremophilic bacterium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.