ArticleBiophysical journal2008
High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
What it found
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Who cites it
125 citing papers in PubMed.
- Single-Molecule 3D Nanoscopy for Application to Cryofixed Samples.The journal of physical chemistry. B · 2025Article
- Simultaneous particle tracking, phase retrieval and point spread function reconstruction.bioRxiv : the preprint server for biology · 2025Article
- Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging.The journal of physical chemistry. B · 2024Article
- BNP-Track: a framework for superresolved tracking.Nature methods · 2024Article
- Long axial-range double-helix point spread functions for 3D volumetric super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
- Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.Nature methods · 2024Article
- Universal inverse modelling of point spread functions for SMLM localization and microscope characterization.bioRxiv : the preprint server for biology · 2023Article
- Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence.Light, science & applications · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- Three-dimensional close-to-substrate trajectories of magnetic microparticles in dynamically changing magnetic field landscapes.Scientific reports · 2022Article
- Super-resolved three-dimensional near-field mapping by defocused imaging and tracking of fluorescent emitters.Nanophotonics (Berlin, Germany) · 2022Article
- Three-dimensional tracking using a single-spot rotating point spread function created by a multiring spiral phase plate.Journal of biomedical optics · 2022Article
- Global fitting for high-accuracy multi-channel single-molecule localization.Nature communications · 2022Article
- Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells.iScience · 2022Article
- Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics.Molecular biology of the cell · 2022Article
- Approach to map nanotopography of cell surface receptors.Communications biology · 2022Article
- Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.Nature communications · 2022Article
- High speed two-photon laser scanning stereomicroscopy for three-dimension tracking multiple particles simultaneously in three-dimension.Frontiers in photonics · 2022Article
- Single Molecules Are Your Quanta: A Bottom-Up Approach toward Multidimensional Super-resolution Microscopy.ACS nano · 2021Review
- Multifocal imaging for precise, label-free tracking of fast biological processes in 3D.Nature communications · 2021Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Single particle tracking in three dimensions in a live cell environment holds the promise of revealing important new biological insights. However, conventional microscopy-based imaging techniques are not well suited for fast three-dimensional (3D) tracking of single particles in cells. Previously we developed an imaging modality multifocal plane microscopy (MUM) to image fast intracellular dynamics in three dimensions in live cells. Here, we introduce an algorithm, the MUM localization algorithm (MUMLA), to determine the 3D position of a point source that is imaged using MUM. We validate MUMLA through simulated and experimental data and show that the 3D position of quantum dots can be determined over a wide spatial range. We demonstrate that MUMLA indeed provides the best possible accuracy with which the 3D position can be determined. Our analysis shows that MUM overcomes the poor depth discrimination of the conventional microscope, and thereby paves the way for high accuracy tracking of nanoparticles in a live cell environment. Here, using MUM and MUMLA we report for the first time the full 3D trajectories of QD-labeled antibody molecules undergoing endocytosis in live cells from the plasma membrane to the sorting endosome deep inside the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.