ArticleNucleic acids research2008
The application of real-time PCR to the analysis of T cell repertoires.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Public and private human T-cell clones respond differentially to HCMV antigen when boosted by CD3 copotentiation.Blood advances · 2020Article
- Single-Cell Sequencing of T cell Receptors: A Perspective on the Technological Development and Translational Application.Advances in experimental medicine and biology · 2020Review
- The early proximal αβ TCR signalosome specifies thymic selection outcome through a quantitative protein interaction network.Science immunology · 2019Article
- Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis.Cell host & microbe · 2017Article
- A novel method for analysis of human T cell repertoires by real-time PCR.Journal of immunological methods · 2014Article
- Vitamin C promotes maturation of T-cells.Antioxidants & redox signaling · 2013Article
- Affinity maturation of antibodies requires integrity of the adult thymus.European journal of immunology · 2012Article
- MHC class II epitope nesting modulates dendritic cell function and improves generation of antigen-specific CD4 helper T cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice.The Journal of clinical investigation · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diversity of T-cell populations is determined by the spectrum of antigen-specific T-cell receptors (TCRs) that are heterodimers of alpha and beta subunits encoded by rearranged combinations of variable (AV and BV), joining (AJ and BJ), and constant region genes (AC and BC). We have developed a novel approach for analysis of beta transcript diversity in mice with a real-time PCR-based method that uses a matrix of BV- and BJ-specific primers to amplify 240 distinct BV-BJ combinations. Defined endpoints (Ct values) and dissociation curves are generated for each BV-BJ combination and the Ct values are consolidated in a matrix that characterizes the beta transcript diversity of each RNA sample. Relative diversities of BV-BJ combinations in individual RNA samples are further described by estimates of scaled entropy. A skin allograft system was used to demonstrate that dissection of repertoires into 240 BV-BJ combinations increases efficiency of identifying and sequencing beta transcripts that are overrepresented at inflammatory sites. These BV-BJ matrices should generate greater investigation in laboratory and clinical settings due to increased throughput, resolution and identification of overrepresented TCR transcripts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.