ArticleBMC cancer2008
Methylation screening of the TGFBI promoter in human lung and prostate cancer by methylation-specific PCR.
Article in BMC cancer, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- Differential expression of TGF-β1, TGF-β2, and TGF-β3 across WHO grades in meningioma: convergent evidence from mRNA, methylation, miRNA, and protein analysis.Journal of neuro-oncology · 2026Article
- Integrated Analysis Identified TGFBI as a Biomarker of Disease Severity and Prognosis Correlated with Immune Infiltrates in Patients with Sepsis.Journal of inflammation research · 2024Article
- Assessment of the TGFB1 gene expression and methylation status of the promoter region in patients with colorectal cancer.Scientific reports · 2022Article
- Downregulation ofGenes & diseases · 2022Article
- Identification of Hub Genes Associated With Sensitivity of 5-Fluorouracil Based Chemotherapy for Colorectal Cancer by Integrated Bioinformatics Analysis.Frontiers in oncology · 2021Article
- Epigenetics in Inflammatory Breast Cancer: Biological Features and Therapeutic Perspectives.Cells · 2020Review
- Epigenetic silencing of TGFBI confers resistance to trastuzumab in human breast cancer.Breast cancer research : BCR · 2019Article
- Article
- Protective effect of stromal Dickkopf-3 in prostate cancer: opposing roles for TGFBI and ECM-1.Oncogene · 2018Article
- Induction of Interferon Kappa in Human Papillomavirus 16 Infection by Transforming Growth Factor Beta-Induced Promoter Demethylation.Journal of virology · 2018Article
- APIM-peptide targeting PCNA improves the efficacy of docetaxel treatment in the TRAMP mouse model of prostate cancer.Oncotarget · 2018Article
- Bigh3 silencing increases retinoblastoma tumor growth in the murine SV40-TAg-Rb model.Oncotarget · 2017Article
- The role of TGFBI (βig-H3) in gastrointestinal tract tumorigenesis.Molecular cancer · 2015Article
- Epigenetic regulation of inflammatory cytokines and associated genes in human malignancies.Mediators of inflammation · 2015Review
- TGFBI expression is an independent predictor of survival in adjuvant-treated lung squamous cell carcinoma patients.British journal of cancer · 2014Article
- βig-h3 promotes human osteosarcoma cells metastasis by interacting with integrin α2β1 and activating PI3K signaling pathway.PloS one · 2014Article
- Hypermethylation of TGF-β1 gene promoter in gastric cancer.World journal of gastroenterology · 2013Article
- TGF-β mediated DNA methylation in prostate cancer.Translational andrology and urology · 2012Article
- Global gene expression changes in human urothelial cells exposed to low-level monomethylarsonous acid.Toxicology · 2012Article
- TGFBI promoter hypermethylation correlating with paclitaxel chemoresistance in ovarian cancer.Journal of experimental & clinical cancer research : CR · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHypermethylation of the TGFBI promoter has been shown to correlate with decreased expression of this gene in human tumor cell lines. In this study, we optimized a methylation-specific polymerase chain reaction (MSP) method and investigated the methylation status of the TGFBI promoter in human lung and prostate cancer specimens.
methodsMethylation-specific primers were designed based on the methylation profiles of the TGFBI promoter in human tumor cell lines, and MSP conditions were optimized for accurate and efficient amplification. Genomic DNA was isolated from lung tumors and prostatectomy tissues of prostate cancer patients, bisulfite-converted, and analyzed by MSP.
resultsAmong 50 lung cancer samples, 44.0% (22/50) harbored methylated CpG sites in the TGFBI promoter. An analysis correlating gene methylation status with clinicopathological cancer features revealed that dense methylation of the TGFBI promoter was associated with a metastatic phenotype, with 42.9% (6/14) of metastatic lung cancer samples demonstrating dense methylation vs. only 5.6% (2/36) of primary lung cancer samples (p < 0.05). Similar to these lung cancer results, 82.0% (41/50) of prostate cancer samples harbored methylated CpG sites in the TGFBI promoter, and dense methylation of the promoter was present in 38.9% (7/18) of prostate cancer samples with the feature of locoregional invasiveness vs. only 19.4% (6/31) of prostate cancer samples without locoregional invasiveness (p < 0.05). Furthermore, promoter hypermethylation correlated with highly reduced expression of the TGFBI gene in human lung and prostate tumor cell lines.
conclusionWe successfully optimized a MSP method for the precise and efficient screening of TGFBI promoter methylation status. Dense methylation of the TGFBI promoter correlated with the extent of TGFBI gene silencing in tumor cell lines and was related to invasiveness of prostate tumors and metastatic status of lung cancer tumors. Thus, TGFBI promoter methylation can be used as a potential prognostic marker for invasiveness and metastasis in prostate and lung cancer patients, respectively.
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