Trial reportCaries research2008
Effects of sucrose on the extracellular matrix of plaque-like biofilm formed in vivo, studied by proteomic analysis.
Trial report in Caries research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06957509 (Salivary Metaproteome as a Prediction Tool in Early Childhood Caries), which is not on this map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Salivary Metaproteome as a Prediction Tool in Early Childhood Caries
Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Engineered Salivary Peptides Reduce Enamel Demineralization Provoked by CariogenicMicroorganisms · 2022Article
- Matrix glycoconjugate characterization in multispecies biofilms and bioaggregates from the environment by means of fluorescently-labeled lectins.Frontiers in microbiology · 2022Article
- A Proteomic Analysis of Discolored Tooth Surfaces after the Use of 0.12% Chlorhexidine (CHX) Mouthwash and CHX Provided with an Anti-Discoloration System (ADS).Materials (Basel, Switzerland) · 2021Article
- The dental plaque biofilm matrix.Periodontology 2000 · 2021Review
- The Inhibitory Effects of Ficin onBioMed research international · 2021Article
- Article
- Adhesion of biofilms on titanium measured by laser-induced spallation.Experimental mechanics · 2019Article
- Biofilm three-dimensional architecture influences in situ pH distribution pattern on the human enamel surface.International journal of oral science · 2017Article
- Fluorescence Lectin Bar-Coding of Glycoconjugates in the Extracellular Matrix of Biofilm and Bioaggregate Forming Microorganisms.Microorganisms · 2017Article
- Devices for In situ Development of Non-disturbed Oral Biofilm. A Systematic Review.Frontiers in microbiology · 2016Review
- The impact of stannous, fluoride ions and its combination on enamel pellicle proteome and dental erosion prevention.PloS one · 2015Article
- Metaproteome analysis of endodontic infections in association with different clinical conditions.PloS one · 2013Article
- A Comparative Study of the Outer Membrane Proteome from an Atypical and a Typical Enteropathogenic Escherichia coli.The open microbiology journal · 2011Article
- Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms.Caries research · 2011Review
- Protein extraction of formalin-fixed, paraffin-embedded tissue enables robust proteomic profiles by mass spectrometry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Enamel Carious Lesion Development in Response to Sucrose and Fluoride Concentrations and to Time of Biofilm Formation: An Artificial-Mouth Study.Journal of oral diseasesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Previous studies have shown that sucrose promotes changes in the composition of the extracellular matrix (ECM) of plaque-like biofilm (PLB), but its effect on protein expression has not been studied in vivo. Therefore, the protein compositions of ECM of PLB formed with and without sucrose exposure were analyzed by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For this purpose, a crossover study was conducted during two phases of 14 days each, during which a volunteer wore a palatal appliance containing eight enamel blocks for PLB accumulation. In each phase, a 20% sucrose solution or distilled and deionized water (control) were extraorally dripped onto the blocks 8x/day. On the 14th day, the PLB were collected, the ECM proteins were extracted, separated by two-dimensional gel electrophoresis, digested by in-gel trypsin and MALDI-TOF MS analyzed. In the ECM of PLB formed under sucrose exposure, the following changes compared with the control PLB were observed: (1) the presence of upregulated proteins that may be involved in bacterial response to environmental changes induced by sucrose and (2) the absence of calcium-binding proteins that may partly explain the low inorganic concentration found in ECM of PLB formed under sucrose exposure. The findings showing that sucrose affected the ECM protein composition of PLB in vivo provide further insight into the unique cariogenic properties of this dietary carbohydrate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.