ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2008
Effect of ethanol on hydrogen peroxide-induced AMPK phosphorylation.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- Hydrogen peroxide sensing and signaling in physiology and disease: implications for therapy.Signal transduction and targeted therapy · 2026Review
- β-Cryptoxanthin Confers Radioprotection against Intestinal Injury via NRF2-Mediated Antioxidant Response and Gut Microbiota Reprogramming.International journal of biological sciences · 2026Article
- Article
- Endogenous ethanol production in health and disease.Nature reviews. Gastroenterology & hepatology · 2024Review
- Pathogenic mechanisms and regulatory factors involved in alcoholic liver disease.Journal of translational medicine · 2023Review
- Ethanol sustains phosphorylated tau protein in the cultured neonatal rat hippocampus: Implications for fetal alcohol spectrum disorders.Alcohol (Fayetteville, N.Y.) · 2022Article
- Betaine in ameliorating alcohol-induced hepatic steatosis.European journal of nutrition · 2022Review
- Beneficial Effects of Betaine: A Comprehensive Review.Biology · 2021Review
- Nutritional Ketosis as a Potential Treatment for Alcohol Use Disorder.Frontiers in psychiatry · 2021Review
- AMPK, Mitochondrial Function, and Cardiovascular Disease.International journal of molecular sciences · 2020Review
- Liver-specific ceramide reduction alleviates steatosis and insulin resistance in alcohol-fed mice.Journal of lipid research · 2020Article
- LB100 ameliorates nonalcoholic fatty liver diseaseWorld journal of gastroenterology · 2019Article
- AMPK mediates inhibition of electrolyte transport and NKCC1 activity by reactive oxygen species.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Effect of ethanol on lipid metabolism.Journal of hepatology · 2019Review
- Role of Transcription Factors in Steatohepatitis and Hypertension after Ethanol: The Epicenter of Metabolism.Biomolecules · 2016Review
- [Role of lipin-1 in the pathogenesis of alcoholic fatty liver disease].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2016Article
- Mechanisms of intrahepatic triglyceride accumulation.World journal of gastroenterology · 2016Review
- NOX1 to NOX2 switch deactivates AMPK and induces invasive phenotype in colon cancer cells through overexpression of MMP-7.Molecular cancer · 2015Article
- [Role of ceramide in the pathogenesis of alcoholic fatty liver disease].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2015Article
- Acute alcohol modulates cardiac function as PI3K/Akt regulates oxidative stress.Alcoholism, clinical and experimental research · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
AMP-activated protein kinase (AMPK) responds to oxidative stress. Previous work has shown that ethanol treatment of cultured hepatoma cells and of mice inhibited the activity of AMPK and reduced the amount of AMPK protein. Ethanol generates oxidative stress in the liver. Since AMPK is activated by reactive oxygen species, it seems paradoxical that ethanol would inhibit AMPK in the hepatoma cells. In an attempt to understand the mechanism whereby ethanol inhibits AMPK, we studied the effect of ethanol on AMPK activation by exogenous hydrogen peroxide. The effects of ethanol, hydrogen peroxide, and inhibitors of protein phosphatase 2A (PP2A) [either okadaic acid or PP2A small interference RNA (siRNA)] on AMPK phosphorylation and activity were examined in rat hepatoma cells (H4IIEC3) and HeLa cells. In H4IIEC3 cells, hydrogen peroxide (H(2)O(2), 1 mM) transiently increased the level of phospho-AMPK to 1.5-fold over control (P < 0.05). Similar findings were observed in HeLa cells, which do not express the upstream AMPK kinase, LKB1. H(2)O(2) markedly increased the phosphorylation of LKB1 in H4IIEC3 cells. Ethanol significantly inhibited the phosphorylation of PKC-zeta, LKB1, and AMPK caused by exposure to H(2)O(2). This inhibitory effect of ethanol required its metabolism. More importantly, the inhibitory effects of ethanol on H(2)O(2)-induced AMPK phosphorylation were attenuated by the presence of the PP2A inhibitor, okadaic acid, or PP2A siRNA. The inhibitory effect of ethanol on AMPK phosphorylation is exerted through the inhibition of PKC-zeta and LKB1 phosphorylation and the activation of PP2A.
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