ArticleThe Journal of physiology2008
A limited contribution of Ca2+ current facilitation to paired-pulse facilitation of transmitter release at the rat calyx of Held.
Article in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 72 citations in OpenAlex.
- Presynaptic CaCell reports · 2026Article
- Non-calyceal inputs gate the timing of calyx of Held evoked MNTB output.Communications biology · 2026Article
- Oxytocin Neuron-Specific CAPS2 Deficiency in Mice Impairs Hippocampal Plasticity and Long-Term Memory Through Reduced ERK Phosphorylation in the Ventral hippocampus.The European journal of neuroscience · 2026Article
- Presynaptic CabioRxiv : the preprint server for biology · 2026Article
- Synaptic Facilitation Enhances the Reliability and Precision of High-Frequency Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Article
- Cellular and synaptic specializations for sub-millisecond precision in the mammalian auditory brainstem.Frontiers in cellular neuroscience · 2025Review
- Alleviation of migraine related pain and anxiety by inhibiting calcium-stimulating AC1-dependent CGRP in the insula of adult rats.The journal of headache and pain · 2024Article
- Interpretation of presynaptic phenotypes of synaptic plasticity in terms of a two-step priming process.The Journal of general physiology · 2024Article
- A sequential two-step priming scheme reproduces diversity in synaptic strength and short-term plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Diverse roles of Synaptotagmin-7 in regulating vesicle fusion.Current opinion in neurobiology · 2020Review
- The Role of CaCell reports · 2019Article
- Underpinning heterogeneity in synaptic transmission by presynaptic ensembles of distinct morphological modules.Nature communications · 2019Article
- Dynamic Factors for Transmitter Release at Small Presynaptic Boutons Revealed by Direct Patch-Clamp Recordings.Frontiers in cellular neuroscience · 2019Review
- Dynamics of volume-averaged intracellular CaThe Journal of physiology · 2017Article
- Review
- Some Subtle Lessons from the Calyx of Held Synapse.Biophysical journal · 2017Review
- The calyx of Held in the auditory system: Structure, function, and development.Hearing research · 2016Review
- Article
- Ca(2+) current facilitation determines short-term facilitation at inhibitory synapses between cerebellar Purkinje cells.The Journal of physiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have suggested that transmitter release facilitation at synapses is largely mediated by presynaptic Ca(2+) current facilitation, but the exact contribution of Ca(2+) current facilitation has not been determined quantitatively. Here, we determine the contribution of Ca(2+) current facilitation, and of an increase in the residual free Ca(2+) concentration ([Ca(2+)](i)) in the nerve terminal, to paired-pulse facilitation of transmitter release at the calyx of Held. Under conditions of low release probability imposed by brief presynaptic voltage-clamp steps, transmitter release facilitation at short interstimulus intervals (4 ms) was 227 +/- 31% of control, Ca(2+) current facilitation was 113 +/- 4% of control, and the peak residual [Ca(2+)](i) was 252 +/- 18 nm over baseline. By inferring the 'local' [Ca(2+)](i) transients that drive transmitter release during these voltage-clamp stimuli with the help of a kinetic release model, we estimate that Ca(2+) current facilitation contributes to approximately 40% to paired-pulse facilitation of transmitter release. The remaining component of facilitation strongly depends on the build-up, and on the decay of the residual free [Ca(2+)](i), but cannot be explained by linear summation of the residual free [Ca(2+)](i), and the back-calculated 'local' [Ca(2+)](i) signal, which only accounts for approximately 10% of the total release facilitation. Further voltage-clamp experiments designed to compensate for Ca(2+) current facilitation demonstrated that about half of the observed transmitter release facilitation remains in the absence of Ca(2+) current facilitation. Our results indicate that paired-pulse facilitation of transmitter release at the calyx of Held is driven by at least two distinct mechanisms: Ca(2+) current facilitation, and a mechanism independent of Ca(2+) current facilitation that closely tracks the time course of residual free [Ca(2+)](i).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.