ArticleGenetics2008
Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant molecular biology · 2024Article
- Comparing the Ability of Secretory Signal Peptides for Heterologous Expression of Anti-Lipopolysaccharide Factor 3 inMarine drugs · 2023Article
- Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (International journal of molecular sciences · 2022Article
- Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants.Nature communications · 2022Article
- Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins.Applied microbiology and biotechnology · 2022Article
- An Improved Top-Down Mass Spectrometry Characterization ofJournal of the American Society for Mass Spectrometry · 2021Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Stochasticity in transcriptional expression of a negative regulator of Arabidopsis ABA network.3 Biotech · 2019Article
- Article
- UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems.Plant molecular biology · 2016Article
- RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker.Plant molecular biology · 2016Article
- Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2015Article
- Reduction in carotenoid levels in the marine diatom Phaeodactylum tricornutum by artificial microRNAs targeted against the endogenous phytoene synthase gene.Marine biotechnology (New York, N.Y.) · 2015Article
- Heat shock factor 1 counteracts epigenetic silencing of nuclear transgenes in Chlamydomonas reinhardtii.Nucleic acids research · 2013Article
- Evidence for a role of VIPP1 in the structural organization of the photosynthetic apparatus in Chlamydomonas.The Plant cell · 2012Article
- Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii.Plant methods · 2011Article
- RNA-mediated silencing in Algae: biological roles and tools for analysis of gene function.Eukaryotic cell · 2011Review
- An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance.Current genetics · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interferences in the unicellular green alga, Chlamydomonas reinhardtii, can be silenced. We have used the silencing of a transgene (aadA) that confers resistance to spectinomycin to investigate the mechanisms responsible for silencing by an artificial inverted repeat (IR) of the aadA gene. The IR construct provided strong silencing, but the RNAi efficiency varied among subclones of a single RNAi-transformed strain with successive cell divisions. Northern blot analyses revealed an inverse correlation between the copy number of the hairpin RNA and the spectinomycin resistance of the subclones. There is an inverse correlation between the efficiency of RNAi and the frequency of methylated CpG (*CpG) in the silenced region. No significant methylated cytosine was observed in the target aadA gene, which suggests the absence of RNA-directed DNA methylation in trans. Several experiments suggest the existence of an intrinsic IR sequence-dependent but a transcription-independent DNA methylation system in C. reinhardtii. The correlation between the *CpG levels and the IR transcript implies the existence of IR DNA-dependent DNA methylation. Treatment of RNAi-induced cells with a histone deacetylase inhibitor, Trichostatin A, rapidly increased the amount of the hairpin RNA and suggests that transcription of the silencer construct was repressed by *CpG-related silencing mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.