Evidence map›Paper›PMID 18832300›Full record

ArticleMicrobiology (Reading, England)2008

Diversity of IncP-9 plasmids of Pseudomonas.

Yanina R Sevastsyanovich, Renata Krasowiak, Lewis E H Bingle, Anthony S Haines, Sergey L Sokolov, Irina A Kosheleva, Anastassia A Leuchuk, Marina A Titok, Kornelia Smalla, Christopher M Thomas

Open access · greenAbstract read
In one paragraph

Article in Microbiology (Reading, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
5.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 57 citations in OpenAlex.

  1. Article
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  5. TnMicroorganisms · 2020
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  15. Conjugative plasmids: vessels of the communal gene pool.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009
    Review
  16. Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 4 countries.

Yanina R SevastsyanovichSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Renata KrasowiakSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Lewis E H BingleSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Anthony S HainesSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Sergey L SokolovSkryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
Irina A KoshelevaSkryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
Anastassia A LeuchukGenetics Department, Biology Faculty, Belarus State University, 6 Kurchatova St, Minsk 220064, Belarus.
Marina A TitokGenetics Department, Biology Faculty, Belarus State University, 6 Kurchatova St, Minsk 220064, Belarus.
Kornelia SmallaJulius Kühn Institute - Federal Research Centre for Cultivated Plants (JKI), Messeweg 11/12, 38104 Braunschweig, Germany.
Christopher M ThomasSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
University of Birmingham · GBBelarusian State University · BYG. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms · RUJulius Kühn-Institut · DE

Funding

Wellcome TrustWellcome Trust 06083Wellcome Trust 067526/Z
6 · The paper itself

Abstract

IncP-9 plasmids are important vehicles for degradation and resistance genes that contribute to the adaptability of Pseudomonas species in a variety of natural habitats. The three completely sequenced IncP-9 plasmids, pWW0, pDTG1 and NAH7, show extensive homology in replication, partitioning and transfer loci (an approximately 25 kb region) and to a lesser extent in the remaining backbone segments. We used PCR, DNA sequencing, hybridization and phylogenetic analyses to investigate the genetic diversity of 30 IncP-9 plasmids as well as the possibility of recombination between plasmids belonging to this family. Phylogenetic analysis of rep and oriV sequences revealed nine plasmid subgroups with 7-35 % divergence between them. Only one phenotypic character was normally associated with each subgroup, except for the IncP-9beta cluster, which included naphthalene- and toluene-degradation plasmids. The PCR and hybridization analysis using pWW0- and pDTG1-specific primers and probes targeting selected backbone loci showed that members of different IncP-9 subgroups have considerable similarity in their overall organization, supporting the existence of a conserved ancestral IncP-9 sequence. The results suggested that some IncP-9 plasmids are the product of recombination between plasmids of different IncP-9 subgroups but demonstrated clearly that insertion of degradative transposons has occurred on multiple occasions, indicating that association of this phenotype with these plasmids is not simply the result of divergent evolution from a single successful ancestral degradative plasmid.

Indexed as

Genetic VariationDNA, BacterialDNA Transposable ElementsEvolution, MolecularGenes, BacterialMolecular Sequence DataPhenotypePhylogenyPlasmidsPolymerase Chain ReactionPseudomonasRecombination, GeneticSequence AlignmentSequence Analysis, DNADNA, BacterialDNA Transposable Elements

Identifiers

PMID18832300
PMCPMC2885752
OpenAlexW2157687101

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.