ArticleJournal of experimental botany2008
Homeostatic control of slow vacuolar channels by luminal cations and evaluation of the channel-mediated tonoplast Ca2+ fluxes in situ.
Article in Journal of experimental botany, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Potassium homeostasis and signalling: from the whole plant to the subcellular level.Quantitative plant biology · 2025Review
- TPC1-Type Channels inPlants (Basel, Switzerland) · 2022Article
- Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles.Stress biology · 2022Review
- Major vacuolar TPC1 channel in stress signaling: what matters, KStress biology · 2022Review
- Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.International journal of molecular sciences · 2021Article
- Mechanisms of Plant Responses and Adaptation to Soil Salinity.Innovation (Cambridge (Mass.)) · 2020Review
- Voltage-dependent gating of SV channel TPC1 confers vacuole excitability.Nature communications · 2019Article
- Physiological, Anatomical and Metabolic Implications of Salt Tolerance in the Halophyte Salvadora persica under Hydroponic Culture Condition.Frontiers in plant science · 2016Article
- Preferential damaging effects of limited magnesium bioavailability on photosystem I in Sulla carnosa plants.Planta · 2015Article
- Calcium Signals from the Vacuole.Plants (Basel, Switzerland) · 2013Review
- Towards the Physics of Calcium Signalling in Plants.Plants (Basel, Switzerland) · 2013Review
- Reduced tonoplast fast-activating and slow-activating channel activity is essential for conferring salinity tolerance in a facultative halophyte, quinoa.Plant physiology · 2013Article
- Differential activity of plasma and vacuolar membrane transporters contributes to genotypic differences in salinity tolerance in a Halophyte Species, Chenopodium quinoa.International journal of molecular sciences · 2013Article
- Modulation of plant TPC channels by polyunsaturated fatty acids.Journal of experimental botany · 2012Article
- Subcellular and single-molecule imaging of plant fluorescent proteins using total internal reflection fluorescence microscopy (TIRFM).Journal of experimental botany · 2011Article
- Calcium efflux systems in stress signaling and adaptation in plants.Frontiers in plant science · 2011Article
- Calcium signals: the lead currency of plant information processing.The Plant cell · 2010Review
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6 authors.
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Abstract
Ca(2+), Mg(2+), and K(+) activities in red beet (Beta vulgaris L.) vacuoles were evaluated using conventional ion-selective microelectrodes and, in the case of Ca(2+), by non-invasive ion flux measurements (MIFE) as well. The mean vacuolar Ca(2+) activity was approximately 0.2 mM. Modulation of the slow vacuolar (SV) channel voltage dependence by Ca(2+) in the absence and presence of other cations at their physiological concentrations was studied by patch-clamp in excised tonoplast patches. Lowering pH at the vacuolar side from 7.5 to 5.5 (at zero vacuolar Ca(2+)) did not affect the channel voltage dependence, but abolished sensitivity to luminal Ca(2+) within a physiological range of concentrations (0.1-1.0 mM). Aggregation of the physiological vacuolar Na(+) (60 mM) and Mg(2+) (8 mM) concentrations also results in the SV channel becoming almost insensitive to vacuolar Ca(2+) variation in a range from nanomoles to 0.1 mM. At physiological cation concentrations at the vacuolar side, cytosolic Ca(2+) activates the SV channel in a voltage-independent manner with K(d)=0.7-1.5 microM. Comparison of the vacuolar Ca(2+) fluxes measured by both the MIFE technique and from estimating the SV channel activity in attached patches, suggests that, at resting membrane potentials, even at elevated (20 microM) cytosolic Ca(2+), only 0.5% of SV channels are open. This mediates a Ca(2+) release of only a few pA per vacuole (approximately 0.1 pA per single SV channel). Overall, our data suggest that the release of Ca(2+) through SV channels makes little contribution to a global cytosolic Ca(2+) signal.
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