Evidence map›Paper›PMID 18832127›Full record

ArticleJournal of clinical microbiology2008

Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism analysis of dnaJ gene.

Tomasz Hauschild, Srdjan Stepanovic

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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  7. Article
  8. Evaluation of discrepancies between oxacillin and cefoxitin susceptibility in coagulase-negative staphylococci.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Tomasz HauschildDepartment of Microbiology, Institute of Biology, University of Bialystok, Bialystok, Poland. thausch@uwb.edu.pl
Srdjan Stepanovic

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method was validated by the identification of 23 clinical staphylococcal strains, and the results were compared with those obtained by other genotypic identification methods. A 100% concordance of the results was shown. Therefore, PCR-RFLP analysis of the dnaJ gene is proposed as a reliable and reproducible method for the identification of Staphylococcus spp.

Indexed as

Polymorphism, Restriction Fragment LengthBacterial ProteinsDNA, BacterialDNA FingerprintingHSP40 Heat-Shock ProteinsHumansPolymerase Chain ReactionSensitivity and SpecificityStaphylococcal InfectionsStaphylococcusBacterial ProteinsDNA, BacterialHSP40 Heat-Shock Proteins

Identifiers

PMID18832127
PMCPMC2593281

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.