ArticleBMC genomics2008
Unassigned MURF1 of kinetoplastids codes for NADH dehydrogenase subunit 2.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Blastocrithidia nonstop mitochondrial genome and its expression are remarkably insulated from nuclear codon reassignment.Nucleic acids research · 2024Article
- Separating the Wheat from the Chaff: RNA Editing and Selection of Translatable mRNA in Trypanosome Mitochondria.Pathogens (Basel, Switzerland) · 2019Review
- Respiratory chain Complex I of unparalleled divergence in diplonemids.The Journal of biological chemistry · 2018Article
- Analysis of the Trypanosoma brucei EATRO 164 Bloodstream Guide RNA Transcriptome.PLoS neglected tropical diseases · 2016Article
- Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen.Parasites & vectors · 2015Article
- Enigmatic presence of mitochondrial complex I in Trypanosoma brucei bloodstream forms.Eukaryotic cell · 2012Article
- Complete set of mitochondrial pan-edited mRNAs in Leishmania mexicana amazonensis LV78.Molecular and biochemical parasitology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn a previous study, we conducted a large-scale similarity-free function prediction of mitochondrion-encoded hypothetical proteins, by which the hypothetical gene murf1 (maxicircle unidentified reading frame 1) was assigned as nad2, encoding subunit 2 of NADH dehydrogenase (Complex I of the respiratory chain). This hypothetical gene occurs in the mitochondrial genome of kinetoplastids, a group of unicellular eukaryotes including the causative agents of African sleeping sickness and leishmaniasis. In the present study, we test this assignment by using bioinformatics methods that are highly sensitive in identifying remote homologs and confront the prediction with available biological knowledge.
resultsComparison of MURF1 profile Hidden Markov Model (HMM) against function-known profile HMMs in Pfam, Panther and TIGR shows that MURF1 is a Complex I protein, but without specifying the exact subunit. Therefore, we constructed profile HMMs for each individual subunit, using all available sequences clustered at various identity thresholds. HMM-HMM comparison of these individual NADH subunits against MURF1 clearly identifies this hypothetical protein as NAD2. Further, we collected the relevant experimental information about kinetoplastids, which provides additional evidence in support of this prediction.
conclusionOur in silico analyses provide convincing evidence for MURF1 being a highly divergent member of NAD2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.