ArticleMolecular biology of the cell2008
The scaffold protein POSH regulates axon outgrowth.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- Mapping Shroom3 expression across the adult mouse.Gene expression patterns : GEP · 2025Article
- Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion.Developmental biology · 2025Review
- Axonal growth inhibitors and their receptors in spinal cord injury: from biology to clinical translation.Neural regeneration research · 2023Review
- The factors affecting neurogenesis after stroke and the role of acupuncture.Frontiers in neurology · 2023Review
- NogoA-expressing astrocytes limit peripheral macrophage infiltration after ischemic brain injury in primates.Nature communications · 2021Article
- Article
- A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology.Biology open · 2021Article
- SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts.Journal of the American Society of Nephrology : JASN · 2018Article
- The pro-apoptotic JNK scaffold POSH/SH3RF1 mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia.Human molecular genetics · 2018Article
- The Involvement of the Myelin-Associated Inhibitors and Their Receptors in CNS Plasticity and Injury.Molecular neurobiology · 2018Review
- TAT-PEP Enhanced Neurobehavioral Functional Recovery by Facilitating Axonal Regeneration and Corticospinal Tract Projection After Stroke.Molecular neurobiology · 2018Article
- Epilepsy and intellectual disability linked protein Shrm4 interaction with GABANature communications · 2017Article
- Targeted inhibition of the Shroom3-Rho kinase protein-protein interaction circumvents Nogo66 to promote axon outgrowth.BMC neuroscience · 2015Article
- Function of Nogo-A/Nogo-A receptor in Alzheimer's disease.CNS neuroscience & therapeutics · 2015Review
- Shroom3 functions downstream of planar cell polarity to regulate myosin II distribution and cellular organization during neural tube closure.Biology open · 2015Article
- The interaction between Shroom3 and Rho-kinase is required for neural tube morphogenesis in mice.Biology open · 2014Article
- Effects of sustained proNGF blockade on attentional capacities in aged rats with compromised cholinergic system.Neuroscience · 2014Article
- Diminished trkA receptor signaling reveals cholinergic-attentional vulnerability of aging.The European journal of neuroscience · 2013Article
- Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.Molecular biology of the cell · 2012Article
- Negative regulation of Yap during neuronal differentiation.Developmental biology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
How scaffold proteins integrate signaling pathways with cytoskeletal components to drive axon outgrowth is not well understood. We report here that the multidomain scaffold protein Plenty of SH3s (POSH) regulates axon outgrowth. Reduction of POSH function by RNA interference (RNAi) enhances axon outgrowth in differentiating mouse primary cortical neurons and in neurons derived from mouse P19 cells, suggesting POSH negatively regulates axon outgrowth. Complementation analysis reveals a requirement for the third Src homology (SH) 3 domain of POSH, and we find that the actomyosin regulatory protein Shroom3 interacts with this domain of POSH. Inhibition of Shroom3 expression by RNAi leads to increased process lengths, as observed for POSH RNAi, suggesting that POSH and Shroom function together to inhibit process outgrowth. Complementation analysis and interference of protein function by dominant-negative approaches suggest that Shroom3 recruits Rho kinase to inhibit process outgrowth. Furthermore, inhibition of myosin II function reverses the POSH or Shroom3 RNAi phenotype, indicating a role for myosin II regulation as a target of the POSH-Shroom complex. Collectively, these results suggest that the molecular scaffold protein POSH assembles an inhibitory complex that links to the actin-myosin network to regulate neuronal process outgrowth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.