ArticleNucleic acids research2008
Testing the palindromic target site model for DNA transposon insertion using the Drosophila melanogaster P-element.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Transposable elements as drivers of genome evolution in Drosophila virilis.Nucleic acids research · 2026Article
- Article
- Whole genome regulatory effect ofmBio · 2024Article
- Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays.Nature structural & molecular biology · 2023Article
- Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network.G3 (Bethesda, Md.) · 2023Article
- Unraveling the palindromic and nonpalindromic motifs of retroviral integration site sequences by statistical mixture models.Genome research · 2023Article
- Article
- Genome ARTIST_v2-An Autonomous Bioinformatics Tool for Annotation of Natural Transposons in Sequenced Genomes.International journal of molecular sciences · 2022Article
- Mechanism and regulation of P element transposition.Open biology · 2020Review
- Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.Nature structural & molecular biology · 2019Article
- An Interscholastic Network To Generate LexA Enhancer Trap Lines inG3 (Bethesda, Md.) · 2019Article
- Article
- Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.Nucleic acids research · 2018Article
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- DNA Transposition at Work.Chemical reviews · 2016Review
- Genome-Wide Analysis of Transposon and Retroviral Insertions Reveals Preferential Integrations in Regions of DNA Flexibility.G3 (Bethesda, Md.) · 2016Article
- Targeting of P-Element Reporters to Heterochromatic Domains by Transposable Element 1360 in Drosophila melanogaster.Genetics · 2016Article
- The casposon-encoded Cas1 protein from Aciduliprofundum boonei is a DNA integrase that generates target site duplications.Nucleic acids research · 2015Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- P Transposable Elements in Drosophila and other Eukaryotic Organisms.Microbiology spectrum · 2015Review
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Understanding the molecular mechanisms that influence transposable element target site preferences is a fundamental challenge in functional and evolutionary genomics. Large-scale transposon insertion projects provide excellent material to study target site preferences in the absence of confounding effects of post-insertion evolutionary change. Growing evidence from a wide variety of prokaryotes and eukaryotes indicates that DNA transposons recognize staggered-cut palindromic target site motifs (TSMs). Here, we use over 10 000 accurately mapped P-element insertions in the Drosophila melanogaster genome to test predictions of the staggered-cut palindromic target site model for DNA transposon insertion. We provide evidence that the P-element targets a 14-bp palindromic motif that can be identified at the primary sequence level, which predicts the local spacing, hotspots and strand orientation of P-element insertions. Intriguingly, we find that the although P-element destroys the complete 14-bp target site upon insertion, the terminal three nucleotides of the P-element inverted repeats complement and restore the original TSM, suggesting a mechanistic link between transposon target sites and their terminal inverted repeats. Finally, we discuss how the staggered-cut palindromic target site model can be used to assess the accuracy of genome mappings for annotated P-element insertions.
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