ArticleNucleic acids research2008
Central base pair flipping and discrimination by PspGI.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Structural basis for recognition of distinct deaminated DNA lesions by endonuclease Q.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.Nucleic acids research · 2019Article
- Behavior of BsoBI endonuclease in the presence and absence of DNA.Journal of molecular modeling · 2017Article
- UbaLAI is a monomeric Type IIE restriction enzyme.Nucleic acids research · 2017Article
- Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.Nucleic acids research · 2017Article
- Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI.Nucleic acids research · 2015Article
- Base flip in DNA studied by molecular dynamics simulationsof differently-oxidized forms of methyl-Cytosine.International journal of molecular sciences · 2014Article
- Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof-of-Principle.The journal of physical chemistry letters · 2014Article
- Restriction endonuclease TseI cleaves A:A and T:T mismatches in CAG and CTG repeats.Nucleic acids research · 2013Article
- The effect of a G:T mispair on the dynamics of DNA.PloS one · 2013Article
- A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota.Frontiers in microbiology · 2013Article
- Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.Nucleic acids research · 2012Article
- Crystal structure and mechanism of action of the N6-methyladenine-dependent type IIM restriction endonuclease R.DpnI.Nucleic acids research · 2012Article
- DNA intercalation without flipping in the specific ThaI-DNA complex.Nucleic acids research · 2011Article
- The type II restriction endonuclease MvaI has dual specificity.Nucleic acids research · 2010Article
- DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme.Nucleic acids research · 2010Article
- Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI.Structure (London, England : 1993) · 2010Article
- Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes.Nucleic acids research · 2009Article
- Structural mechanisms for the 5'-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII.Nucleic acids research · 2009Article
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Authors and funding
8 authors.
Funding
Abstract
PspGI is a representative of a group of restriction endonucleases that recognize a pentameric sequence related to CCNGG. Unlike the previously investigated Ecl18kI, which does not have any specificity for the central base pair, PspGI prefers A/T over G/C in its target site. Here, we present a structure of PspGI with target DNA at 1.7 A resolution. In this structure, the bases at the center of the recognition sequence are extruded from the DNA and flipped into pockets of PspGI. The flipped thymine is in the usual anti conformation, but the flipped adenine takes the normally unfavorable syn conformation. The results of this and the accompanying manuscript attribute the preference for A/T pairs over G/C pairs in the flipping position to the intrinsically lower penalty for flipping A/T pairs and to selection of the PspGI pockets against guanine and cytosine. Our data show that flipping can contribute to the discrimination between normal bases. This adds a new role to base flipping in addition to its well-known function in base modification and DNA damage repair.
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