ArticleBiology of reproduction2009
Segmental expression of the bradykinin type 2 receptor in rat efferent ducts and epididymis and its role in the regulation of aquaporin 9.
Article in Biology of reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Advancement and Potential Applications of Epididymal Organoids.Biomolecules · 2024Review
- Article
- Epididymal epithelial degeneration and lipid metabolism impairment account for male infertility in occludin knockout mice.Frontiers in endocrinology · 2022Article
- The Bdkrb2 gene family provides a novel view of viviparity adaptation in Sebastes schlegelii.BMC ecology and evolution · 2021Article
- Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Animal reproduction · 2021Article
- Whole genome sequencing identifies allelic ratio distortion in sperm involving genes related to spermatogenesis in a swine model.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- Function and therapeutic potential of G protein-coupled receptors in epididymis.British journal of pharmacology · 2020Review
- The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost.Journal of anatomy · 2018Article
- Key factors enhancing sperm fertilizing ability are transferred from the epididymis to the spermatozoa via epididymosomes in the domestic cat model.Journal of assisted reproduction and genetics · 2018Article
- Rats undernourished in utero have altered Ca2+ signaling and reduced fertility in adulthood.Physiological reports · 2015Article
- Characterisation of Lyzls in mice and antibacterial properties of human LYZL6.Asian journal of andrology · 2013Article
- Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis.American journal of physiology. Cell physiology · 2013Article
- Estrogen, efferent ductules, and the epididymis.Biology of reproduction · 2011Review
- Localization and androgen regulation of metastasis-associated protein 1 in mouse epididymis.PloS one · 2010Article
- Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones.Reproduction (Cambridge, England) · 2010Article
- Testosterone upregulates anion secretion across porcine vas deferens epithelia in vitro.Biology of reproduction · 2009Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
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Abstract
Water and solute transport in the efferent ducts and epididymis are important for the establishment of the appropriate luminal environment for sperm maturation and storage. Aquaporin 9 (AQP9) is the main water channel in the epididymis, but its regulation is still poorly understood. Components of the kinin-kallikrein system (KKS), leading to the production of bradykinin (BK), are highly expressed in the lumen of the male reproductive tract. We report here that the epididymal luminal fluid contains a significant amount of BK (2 nM). RT-PCR performed on epididymal epithelial cells isolated by laser capture microdissection (LCM) showed abundant BK type 2 receptor (Bdkrb2) mRNA expression but no type 1 receptor (Bdkrb1). Double-immunofluorescence staining for BDKRB2 and the anion exchanger AE2 (a marker of efferent duct ciliated cells) or the V-ATPase E subunit, official symbol ATP6V1E1 (a marker of epididymal clear cells), showed that BDKRB2 is expressed in the apical pole of nonciliated cells (efferent ducts) and principal cells (epididymis). Triple labeling for BDKRB2, AQP9, and ATP6V1E1 showed that BDKRB2 and AQP9 colocalize in the apical stereocilia of principal cells in the cauda epididymidis. While uniform Bdkrb2 mRNA expression was detected in the efferent ducts and along the epididymal tubule, marked variations were detected at the protein level. BDKRB2 was highest in the efferent ducts and cauda epididymidis, intermediate in the distal initial segment, moderate in the corpus, and undetectable in the proximal initial segment and the caput. Functional assays on tubules isolated from the distal initial segments showed that BK significantly increased AQP9-dependent glycerol apical membrane permeability. This effect was inhibited by BAPTA-AM, demonstrating the participation of calcium in this process. This study, therefore, identifies BK as an important regulator of AQP9.
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