ArticleVeterinary research
Faecal shedding, alimentary clearance and intestinal spread of prions in hamsters fed with scrapie.
Article in Veterinary research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Review
- Detection of Pathognomonic Biomarker PrPBiomolecules · 2020Review
- Early preclinical detection of prions in the skin of prion-infected animals.Nature communications · 2019Article
- Specificity, Size, and Frequency of Spaces That Characterize the Mechanism of Bulk Transepithelial Transport of Prions in the Nasal Cavities of Hamsters and Mice.Journal of virology · 2016Article
- Enhanced prion detection in biological samples by magnetic particle extraction and real-time quaking-induced conversion.The Journal of general virology · 2016Article
- Early and Non-Invasive Detection of Chronic Wasting Disease Prions in Elk Feces by Real-Time Quaking Induced Conversion.PloS one · 2016Article
- Axonal and transynaptic spread of prions.Journal of virology · 2014Article
- Procedures for identifying infectious prions after passage through the digestive system of an avian species.Journal of visualized experiments : JoVE · 2013Article
- Prion diseases as transmissible zoonotic diseases.Osong public health and research perspectives · 2013Review
- Rapid transepithelial transport of prions following inhalation.Journal of virology · 2012Article
- Accelerated shedding of prions following damage to the olfactory epithelium.Journal of virology · 2012Article
- Prion remains infectious after passage through digestive system of American crows (Corvus brachyrhynchos).PloS one · 2012Article
- Assessing prion infectivity of human urine in sporadic Creutzfeldt-Jakob disease.Emerging infectious diseases · 2012Article
- Detection and control of prion diseases in food animals.ISRN veterinary science · 2012Article
- Prion uptake in the gut: identification of the first uptake and replication sites.PLoS pathogens · 2011Article
- Chronic wasting disease prion trafficking via the autonomic nervous system.The American journal of pathology · 2011Article
- Initial fate of prions upon peripheral infection: half-life, distribution, clearance, and tissue uptake.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Detection of prions in the faeces of sheep naturally infected with classical scrapie.Veterinary research · 2011Article
- BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle.Veterinary research · 2011Article
- Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shedding of prions via faeces may be involved in the transmission of contagious prion diseases. Here, we fed hamsters 10mg of 263K scrapie brain homogenate and examined the faecal excretion of disease-associated prion protein (PrP(TSE)) during the course of infection. The intestinal fate of ingested PrP(TSE) was further investigated by monitoring the deposition of the protein in components of the gut wall using immunohistochemistry and paraffin-embedded tissue (PET) blotting. Western blotting of faecal extracts showed shedding of PrP(TSE) in the excrement at 24-72 h post infection (hpi), but not at 0-24 hpi or at later preclinical or clinical time points. About 5% of the ingested PrP(TSE) were excreted via the faeces. However, the bulk of PrP(TSE) was cleared from the alimentary canal, most probably by degradation, while an indiscernible proportion of the inoculum triggered intestinal infection. Components of the gut-associated lymphoid tissue (GALT) and the enteric nervous system (ENS) showed progressing accumulation of PrP(TSE) from 30 days post infection (dpi) and 60 dpi, respectively. At the clinical stage of disease, substantial deposits of PrP(TSE) were found in the GALT in close vicinity to the intestinal lumen. Despite an apparent possibility of shedding from Peyer's patches that may involve the follicle-associated epithelium (FAE), only small amounts of PrP(TSE) were detected in faeces from clinically infected animals by serial protein misfolding cyclic amplification (sPMCA). Although excrement may thus provide a vehicle for the release of endogenously formed PrP(TSE), intestinal clearance mechanisms seem to partially counteract such a mode of prion dissemination.
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