ArticleClinical and vaccine immunology : CVI2008
Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 95 citations in OpenAlex.
- Trichinellosis: A zoonosis that still requires vigilance.PLoS neglected tropical diseases · 2026Review
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- Serological testing forFood and waterborne parasitology · 2022Article
- Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.PLoS neglected tropical diseases · 2021Article
- Second outbreak of Trichinella pseudospiralis in Europe: clinical patterns, epidemiological investigation and identification of the etiological agent based on the western blot patterns of the patients' serum.Zoonoses and public health · 2021Article
- Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring ofInternational journal of nanomedicine · 2021Article
- Human Outbreak of Trichinellosis Caused by Trichinella papuae Nematodes, Central Kampong Thom Province, Cambodia.Emerging infectious diseases · 2020Article
- Anisakis Sensitization in the Croatian fish processing workers: Behavioral instead of occupational risk factors?PLoS neglected tropical diseases · 2020Article
- Article
- International Commission on Trichinellosis: Recommendations on the use of serological tests for the detection ofFood and waterborne parasitology · 2019Review
- Retrospective analysis of hospital discharge records for cases of trichinellosis does not allow evaluation of disease burden in Italy.Parasite (Paris, France) · 2019Article
- Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot.Parasites & vectors · 2018Article
- Seroprevalence of Selected Zoonotic Agents among Hunters from Eastern Poland.Polish journal of microbiology · 2018Article
- Serological tools for detection ofOne health (Amsterdam, Netherlands) · 2016Review
- New diagnostic antigens for early trichinellosis: the excretory-secretory antigens of Trichinella spiralis intestinal infective larvae.Parasitology research · 2015Article
- Early serodiagnosis of trichinellosis by ELISA using excretory-secretory antigens of Trichinella spiralis adult worms.Parasites & vectors · 2015Article
- Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera.Parasites & vectors · 2015Article
- Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis.PLoS neglected tropical diseases · 2014Article
- Identification of early diagnostic antigens from major excretory-secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics.Parasites & vectors · 2014Article
- Patterns and risks of trichinella infection in humans and pigs in northern Laos.PLoS neglected tropical diseases · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trichinellosis is a zoonotic disease caused by the consumption of raw or semiraw meat from different animals harboring Trichinella larvae in their muscles. Since there are no pathognomonic signs, diagnosis can be difficult; for this reason, serology is important. The objective of this study was to validate an enzyme-linked immunosorbent assay (ELISA) using excretory/secretory antigens to detect anti-Trichinella immunoglobulin G antibodies in human sera. A total of 3,505 human serum samples were tested. A receiver-operator characteristic (ROC) curve analysis was performed. The accuracy of the test was determined by calculating the area under the curve, which was equal to 0.999, indicating high accuracy. The coefficient of variation calculated for data from four serum samples in eight working sessions was no higher than 5% for the positive sera or 14% for the negative sera. Moreover, the analysis of the differences in optical density between duplicates indicated a high repeatability for the ELISA. At the ROC optimized cutoff, the sensitivity and specificity of the test were, respectively, 99.2% and 90.6% (specificity of 95.6% when excluding the samples from multiparasitized persons from Tanzania). The validated ELISA showed good performance in terms of sensitivity, repeatability, and reproducibility, whereas the specificity was limited. These results suggest that this test is suitable for detecting anti-Trichinella antibodies in human sera for diagnostic purposes, whereas its use in epidemiological surveys could be questionable.
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