ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2009
Isolation and functional characterization of a stable complex between photoactivated rhodopsin and the G protein, transducin.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Human nonvisual opsin 3 regulates pigmentation of epidermal melanocytes through functional interaction with melanocortin 1 receptor.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Retinal-chitosan Conjugates Effectively Deliver Active Chromophores to Retinal Photoreceptor Cells in Blind Mice and Dogs.Molecular pharmacology · 2018Article
- Palmitoylation is a prerequisite for dimerization-dependent raftophilicity of rhodopsin.The Journal of biological chemistry · 2017Article
- Animals deficient in C2Orf71, an autosomal recessive retinitis pigmentosa-associated locus, develop severe early-onset retinal degeneration.Human molecular genetics · 2015Article
- Chemistry and biology of the initial steps in vision: the Friedenwald lecture.Investigative ophthalmology & visual science · 2014Article
- Crystallization scale preparation of a stable GPCR signaling complex between constitutively active rhodopsin and G-protein.PloS one · 2014Article
- From atomic structures to neuronal functions of g protein-coupled receptors.Annual review of neuroscience · 2013Review
- Asymmetry of the rhodopsin dimer in complex with transducin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Article
- Conformational dynamics of activation for the pentameric complex of dimeric G protein-coupled receptor and heterotrimeric G protein.Structure (London, England : 1993) · 2012Article
- Rhodopsin-transducin heteropentamer: three-dimensional structure and biochemical characterization.Journal of structural biology · 2011Article
- Preparation of an activated rhodopsin/transducin complex using a constitutively active mutant of rhodopsin.Biochemistry · 2011Article
- Coupling efficiency of rhodopsin and transducin in bicelles.Biochemistry · 2011Article
- Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding.The Journal of biological chemistry · 2011Article
- Oligomeric forms of G protein-coupled receptors (GPCRs).Trends in biochemical sciences · 2010Article
- Electrostatic compensation restores trafficking of the autosomal recessive retinitis pigmentosa E150K opsin mutant to the plasma membrane.The Journal of biological chemistry · 2010Article
- Complexes between photoactivated rhodopsin and transducin: progress and questions.The Biochemical journal · 2010Review
- Structural and kinetic modeling of an activating helix switch in the rhodopsin-transducin interface.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Phospholipids are needed for the proper formation, stability, and function of the photoactivated rhodopsin-transducin complex.Biochemistry · 2009Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Transitory binding between photoactivated rhodopsin (Rho* or Meta II) and the G protein transducin (Gt-GDP) is the first step in the visual signaling cascade. Light causes photoisomerization of the 11-cis-retinylidene chromophore in rhodopsin (Rho) to all-trans-retinylidene, which induces conformational changes that allow Gt-GDP to dock onto the Rho* surface. GDP then dissociates from Gt, leaving a transient nucleotide-empty Rho*-Gt(e) complex before GTP becomes bound, and Gt-GTP then dissociates from Rho*. Further biochemical advances are required before structural studies of the various Rho*-Gt complexes can be initiated. Here, we describe the isolation of n-dodecyl-beta-maltoside solubilized, stable, functionally active, Rho*-Gt(e), Rho(e)*-Gt(e), and 9-cis-retinal/11-cis-retinal regenerated Rho-Gt(e) complexes by sucrose gradient centrifugation. In these complexes, Rho* spectrally remained in its Meta II state, and Gt(e) retained its ability to interact with GTPgammaS. Removal of all-trans-retinylidene from Rho*-Gt(e) had no effect on the stability of the Rho(e)*-Gt(e) complex. Moreover, opsin in the Rho(e)*-Gt(e) complex with an empty nucleotide-binding pocket in Gt and an empty retinoid-binding pocket in Rho was regenerated up to 75% without complex dissociation. These results indicate that once Rho* couples with Gt, the chromophore plays a minor role in stabilizing this complex. Moreover, in complexes regenerated with 9-cis-retinal/11-cis-retinal, Rho retains a conformation similar to Rho* that is stabilized by Gt(e) apo-protein.
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