ArticlePlant methods2008
Mutational optimization of the coelenterazine-dependent luciferase from Renilla.
Article in Plant methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
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- Advanced Bioluminescence Reporter with EngineeredBiosensors · 2024Article
- Molecular Dynamics Simulation Combined with Neural Relationship Inference and Markov Model to Reveal the Relationship between Conformational Regulation and Bioluminescence Properties ofMolecules (Basel, Switzerland) · 2024Article
- Exploring the Potential of Arginine to Increase Coelenterazine-Renilla Luciferase Affinity and Enzyme Stability: Kinetic and Molecular Dynamics Studies.The protein journal · 2024Article
- Uncovering the role of sorbitol inBiochemistry and biophysics reports · 2024Article
- Attenuation of phytofungal pathogenicity of Ascomycota by autophagy modulators.Nature communications · 2024Article
- Pseudo-Luciferase Activity of the SARS-CoV-2 Spike Protein forACS central science · 2024Article
- Bioluminescent Imaging Systems for Assay Developments.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- Optical approaches for single-cell and subcellular analysis of GPCR-G protein signaling.Analytical and bioanalytical chemistry · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Imaging extracellular vesicles: current and emerging methods.Journal of biomedical science · 2018Review
- Plasma membrane phosphatidylinositol 4-phosphate and 4,5-bisphosphate determine the distribution and function of K-Ras4B but not H-Ras proteins.The Journal of biological chemistry · 2017Article
- Thermostable exoshells fold and stabilize recombinant proteins.Nature communications · 2017Article
- A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence.Open biology · 2017Article
- Investigation of the fate of type I angiotensin receptor after biased activation.Molecular pharmacology · 2015Article
- Measurement of inositol 1,4,5-trisphosphate in living cells using an improved set of resonance energy transfer-based biosensors.PloS one · 2015Article
- Altered agonist sensitivity of a mutant v2 receptor suggests a novel therapeutic strategy for nephrogenic diabetes insipidus.Molecular endocrinology (Baltimore, Md.) · 2014Article
- Differential processing of Arabidopsis ubiquitin-like Atg8 autophagy proteins by Atg4 cysteine proteases.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Improved methodical approach for quantitative BRET analysis of G Protein Coupled Receptor dimerization.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one hundred molecules of coelenterazine substrate. Mutagenesis of active site residue Pro220 extended the half-life of photon emission, yielding brighter luminescence in E. coli. Random mutagenesis uncovered two new mutations that stabilized and increased photon emission in vivo and in vitro, while ameliorating substrate inhibition. Further amended with a previously identified mutation, a new triple mutant showed a threefold improved kcat, as well as elevated luminescence in Arabidopsis. This advances the utility of RLUC as a reporter protein, biosensor, or resonance energy donor.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.