ArticleThe Plant journal : for cell and molecular biology2009
Effective, homogeneous and transient interference with cytosine methylation in plant genomic DNA by zebularine.
Article in The Plant journal : for cell and molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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Who cites it
67 citing papers in PubMed, 130 citations in OpenAlex.
- Stable Transgenerational Epimutants in Okra Induced by Sodium Butyrate: A Novel Pathway to Functional Breeding.Biochemical genetics · 2026Article
- Ecotype-specific sensitivity to DNA methylation inhibitors in Arabidopsis cmt3 mutants.Molecular genetics and genomics : MGG · 2026Article
- A dynamic epigenetic perspective on above- and belowground phenotypic responses to drought: Insights from global DNA methylation in Erodium cicutarium.Plant biology (Stuttgart, Germany) · 2026Article
- Unraveling novel mechanisms controlling heterosis in seeds: advances and biotechnological applications in crops.Journal of experimental botany · 2025Review
- Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage.BMC plant biology · 2025Article
- Article
- Testing the potential of zebularine to induce heritable changes in crop growth and development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis.Nucleic acids research · 2024Article
- Plasticity and associated epigenetic mechanisms play a role in thermal evolution during range expansion.Evolution letters · 2024Article
- An endogenous promoterFrontiers in plant science · 2024Article
- A tool for rapid, automated characterization of population epigenomics in plants.Scientific reports · 2023Article
- Revealing the transitory and local effect of zebularine on development and on proteome dynamics ofFrontiers in plant science · 2023Article
- Epigenetic Stress and Long-Read cDNA Sequencing of Sunflower (Plants (Basel, Switzerland) · 2022Article
- Experimentally heat-induced transposition increases drought tolerance in Arabidopsis thaliana.The New phytologist · 2022Article
- Transgenerationally Transmitted DNA Demethylation of a Spontaneous Epialleles Using CRISPR/dCas9-TET1cd Targeted Epigenetic Editing in Arabidopsis.International journal of molecular sciences · 2022Article
- Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells.Genes · 2022Article
- Transcriptome, metabolome and suppressor analysis reveal an essential role for the ubiquitin-proteasome system in seedling chloroplast development.BMC plant biology · 2022Article
- Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis.The Plant cell · 2022Article
- Harnessing epigenetic variability for crop improvement: current status and future prospects.Genes & genomics · 2022Review
- Zebularine induces enzymatic DNA-protein crosslinks in 45S rDNA heterochromatin of Arabidopsis nuclei.Nucleic acids research · 2022Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Covalent modification by methylation of cytosine residues represents an important epigenetic hallmark. While sequence analysis after bisulphite conversion allows correlative analyses with single-base resolution, functional analysis by interference with DNA methylation is less precise, due to the complexity of methylation enzymes and their targets. A cytidine analogue, 5-azacytidine, is frequently used as an inhibitor of DNA methyltransferases, but its rapid degradation in aqueous solution is problematic for culture periods of longer than a few hours. Application of zebularine, a more stable cytidine analogue with a similar mode of action that is successfully used as a methylation inhibitor in Neurospora and mammalian tumour cell lines, can significantly reduce DNA methylation in plants in a dose-dependent and transient manner independent of sequence context. Demethylation is connected with transcriptional reactivation and partial decondensation of heterochromatin. Zebularine represents a promising new and versatile tool for investigating the role of DNA methylation in plants with regard to transcriptional control, maintenance and formation of (hetero-) chromatin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.