ArticleThe Journal of cell biology2008
The role of GTP binding and hydrolysis at the atToc159 preprotein receptor during protein import into chloroplasts.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Functional Interaction Between Toc75 POTRA1 Domain and Tic22-III in the Intermembrane Space During Chloroplast Protein Import.Plant direct · 2026Article
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- Molecular Topology of the Transit Peptide during Chloroplast Protein Import.The Plant cell · 2018Article
- The novel chloroplast outer membrane kinase KOC1 is a required component of the plastid protein import machinery.The Journal of biological chemistry · 2017Article
- Chloroplast Preproteins Bind to the Dimer Interface of the Toc159 Receptor during Import.Plant physiology · 2017Article
- Article
- New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.Journal of molecular biology · 2015Review
- The similarity between N-terminal targeting signals for protein import into different organelles and its evolutionary relevance.Frontiers in physiology · 2015Review
- Targeting and assembly of components of the TOC protein import complex at the chloroplast outer envelope membrane.Frontiers in plant science · 2014Review
- Biogenesis and homeostasis of chloroplasts and other plastids.Nature reviews. Molecular cell biology · 2013Review
- Energetic manipulation of chloroplast protein import and the use of chemical cross-linkers to map protein-protein interactions.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Common ground for protein translocation: access control for mitochondria and chloroplasts.Nature reviews. Molecular cell biology · 2011Review
- The molecular basis for distinct pathways for protein import into Arabidopsis chloroplasts.The Plant cell · 2010Article
- Distinct pathways mediate the sorting of tail-anchored proteins to the plastid outer envelope.PloS one · 2010Article
- The acidic domains of the Toc159 chloroplast preprotein receptor family are intrinsically disordered protein domains.BMC biochemistry · 2009Article
- Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts.The Journal of biological chemistry · 2009Article
- A toc159 import receptor mutant, defective in hydrolysis of GTP, supports preprotein import into chloroplasts.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The majority of nucleus-encoded chloroplast proteins are targeted to the organelle by direct binding to two membrane-bound GTPase receptors, Toc34 and Toc159. The GTPase activities of the receptors are implicated in two key import activities, preprotein binding and driving membrane translocation, but their precise functions have not been defined. We use a combination of in vivo and in vitro approaches to study the role of the Toc159 receptor in the import reaction. We show that atToc159-A864R, a receptor with reduced GTPase activity, can fully complement a lethal insertion mutation in the ATTOC159 gene. Surprisingly, the atToc159-A864R receptor increases the rate of protein import relative to wild-type receptor in isolated chloroplasts by stabilizing the formation of a GTP-dependent preprotein binding intermediate. These data favor a model in which the atToc159 receptor acts as part of a GTP-regulated switch for preprotein recognition at the TOC translocon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.