ArticleJournal of molecular biology2008
Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
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- Actinobacterial chalkophores: the biosynthesis of hazimycins.The Journal of antibiotics · 2024Article
- Isonitrile biosynthesis by non-heme iron(II)-dependent oxidases/decarboxylases.Methods in enzymology · 2024Article
- Elucidation of divergent desaturation pathways in the formation of vinyl isonitrile and isocyanoacrylate.Nature communications · 2022Article
- Biosynthesis of Isonitrile- and Alkyne-Containing Natural Products.Annual review of chemical and biomolecular engineering · 2022Review
- Current Understanding toward Isonitrile Group Biosynthesis and Mechanism.Chinese journal of chemistry · 2021Article
- Biocatalysts from cyanobacterial hapalindole pathway afford antivirulent isonitriles against MRSA.Journal of biosciences · 2021Article
- The evolution of virulence inProceedings. Biological sciences · 2020Article
- Studies on the Properties of the Sporulation Specific Protein Dit1 and its Product Formyl Tyrosine.Journal of fungi (Basel, Switzerland) · 2020Article
- Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase.Angewandte Chemie (International ed. in English) · 2018Article
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- Biosynthesis of isonitrile lipopeptides by conserved nonribosomal peptide synthetase gene clusters in Actinobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Design, synthesis and microbiological evaluation of ampicillin-tetramic acid hybrid antibiotics.The Journal of antibiotics · 2017Article
- An iron detection system determines bacterial swarming initiation and biofilm formation.Scientific reports · 2016Article
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- Comparative analysis of hapalindole, ambiguine and welwitindolinone gene clusters and reconstitution of indole-isonitrile biosynthesis from cyanobacteria.BMC microbiology · 2014Article
- Microbial tailoring of acyl peptidic siderophores.Biochemistry · 2014Article
- The pvc operon regulates the expression of the Pseudomonas aeruginosa fimbrial chaperone/usher pathway (cup) genes.PloS one · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The pvcABCD operon of Pseudomonas aeruginosa encodes four proteins (PA2254, PA2255, PA2256, and PA2257) that form a cluster that is responsible for the synthesis of a cyclized isocyano derivative of tyrosine. These proteins, which were identified originally as being responsible for a step in the maturation of the chromophore of the peptide siderophore pyoverdine, have been identified recently as belonging to a family of proteins that produce small organic isonitriles. We report that strains harboring a disruption in the pvcA or pvcB genes are able to grow in iron-depleted conditions and to produce pyoverdine. Additionally, we have determined the three-dimensional crystal structures of PvcA and PvcB. The structure of PvcA demonstrates a novel enzyme architecture that is built upon a Rossmann fold. We have analyzed the sequence conservation of enzymes within this family and identified six conserved motifs. These regions of the protein cluster around a putative active site cavity. The structure of the PvcB protein confirms it is a member of the Fe2+/alpha-ketoglutarate-dependent oxygenase family of enzymes. The active site of PvcB is compared to the structures of other family members and suggests that a conformational change to order several loops will accompany the binding of ligands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.