ArticleJournal of immunological methods2008
Serial culture of murine primary airway epithelial cells and ex vivo replication of human rhinoviruses.
Article in Journal of immunological methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Integrin-β4 regulates the dynamic changes of phenotypic characteristics in association with epithelial-mesenchymal transition (EMT) and RhoA activity in airway epithelial cells during injury and repair.International journal of biological sciences · 2022Article
- Airway epithelial ITGB4 deficiency in early life mediates pulmonary spontaneous inflammation and enhanced allergic immune response.Journal of cellular and molecular medicine · 2020Article
- A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture.Scientific reports · 2018Article
- Temperature-dependent innate defense against the common cold virus limits viral replication at warm temperature in mouse airway cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cystic fibrosis transmembrane conductance regulator dysfunction in VIP knockout mice.American journal of physiology. Cell physiology · 2014Article
- Regulation of IL-4 receptor signaling by STUB1 in lung inflammation.American journal of respiratory and critical care medicine · 2014Article
- Sialyltransferase ST3Gal-III regulates Siglec-F ligand formation and eosinophilic lung inflammation in mice.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- In vitro isolation and cultivation of rabbit tracheal epithelial cells using tissue explant technique.In vitro cellular & developmental biology. Animal · 2013Article
- Loss-of-function of inositol polyphosphate-4-phosphatase reversibly increases the severity of allergic airway inflammation.Nature communications · 2012Article
- Rhinoviruses, allergic inflammation, and asthma.Immunological reviews · 2011Review
- Rhinovirus and the initiation of asthma.Current opinion in allergy and clinical immunology · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Human rhinoviruses (HRV) are the primary etiological agents in cold infections, and represent a serious risk to individuals with chronic respiratory disease such as asthma. In order to develop treatment options for HRV infections, murine models are a crucial component in the study of infection mechanisms due to the wide array of reagents and techniques available to study murine immunology. We present here a cell culture system for studying isolated murine epithelial cell responses to HRV. Monolayers of primary mouse airway epithelial cells were maintained in a serial culture system, and the identity and purity of the cell population was confirmed via immunostaining (positive for cytokeratin, negative for vimentin). Infection of these cells with a minor group rhinovirus (HRV-1A) was evidenced by increases in viral RNA, de novo synthesis of viral proteins, and production of infectious virus. This model will be useful in experiments to define mechanisms of viral replication and host/virus interactions within airway epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.