ArticleBiomaterials2008
Culture on electrospun polyurethane scaffolds decreases atrial natriuretic peptide expression by cardiomyocytes in vitro.
Article in Biomaterials, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 104 citations in OpenAlex.
- Mechanobiology-Driven Metabolic Reprogramming: Integrative Roles of YAP/TAZ Signaling and Extracellular Matrix Dynamics.Cell biology international · 2026Review
- Review
- 3D models of dilated cardiomyopathy: Shaping the chemical, physical and topographical properties of biomaterials to mimic the cardiac extracellular matrix.Bioactive materials · 2022Review
- Novel Poly(ester urethane urea)/Polydioxanone Blends: Electrospun Fibrous Meshes and Films.Molecules (Basel, Switzerland) · 2021Article
- Cardiac Extracellular Matrix as a Platform for Heart Organ Bioengineering: Design and Development of Tissue-Engineered Heart.Advances in experimental medicine and biology · 2021Article
- Electrospun Water-Borne Polyurethane Nanofibrous Membrane as a Barrier for Preventing Postoperative Peritendinous Adhesion.International journal of molecular sciences · 2019Article
- Cardiac tissue engineering: current state-of-the-art materials, cells and tissue formation.Einstein (Sao Paulo, Brazil) · 2018Review
- Interfacial tissue engineering of heart regenerative medicine based on soft cell-porous scaffolds.Journal of thoracic disease · 2018Review
- Tissue Engineering Strategies for Myocardial Regeneration: Acellular Versus Cellular Scaffolds?Tissue engineering. Part B, Reviews · 2016Review
- Tissue engineering the cardiac microenvironment: Multicellular microphysiological systems for drug screening.Advanced drug delivery reviews · 2016Review
- Opportunities for multicomponent hybrid hydrogels in biomedical applications.Biomacromolecules · 2015Review
- Optimization of fully aligned bioactive electrospun fibers for "in vitro" nerve guidance.Journal of materials science. Materials in medicine · 2014Article
- Tough and flexible CNT-polymeric hybrid scaffolds for engineering cardiac constructs.Biomaterials · 2014Article
- A porous tissue engineering scaffold selectively degraded by cell-generated reactive oxygen species.Biomaterials · 2014Article
- PGS:Gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues.Biomaterials · 2013Article
- Nanotopography-guided tissue engineering and regenerative medicine.Advanced drug delivery reviews · 2013Review
- The effect of topography on differentiation fates of matrigel-coated mouse embryonic stem cells cultured on PLGA nanofibrous scaffolds.Tissue engineering. Part A · 2012Article
- Materials fabrication from Bombyx mori silk fibroin.Nature protocols · 2011Article
- The stimulation of the cardiac differentiation of mesenchymal stem cells in tissue constructs that mimic myocardium structure and biomechanics.Biomaterials · 2011Article
- A 3D aligned microfibrous myocardial tissue construct cultured under transient perfusion.Biomaterials · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The function of the mammalian heart depends on the functional alignment of cardiomyocytes, and controlling cell alignment is an important consideration in biomaterial design for cardiac tissue engineering and research. The physical cues that guide functional cell alignment in vitro and the impact of substrate-imposed alignment on cell phenotype, however, are only partially understood. In this report, primary cardiac ventricular cells were grown on electrospun, biodegradable polyurethane (ES-PU) with either aligned or unaligned microfibers. ES-PU scaffolds supported high-density cultures and cell subpopulations remained intact over two weeks in culture. ES-PU cultures contained electrically-coupled cardiomyocytes with connexin-43 localized to points of cell:cell contact. Multi-cellular organization correlated with microfiber orientation and aligned materials yielded highly oriented cardiomyocyte groupings. Atrial natriuretic peptide, a molecular marker that shows decreasing expression during ventricular cell maturation, was significantly lower in cultures grown on ES-PU scaffolds than in those grown on tissue culture polystyrene. Cells grown on aligned ES-PU had significantly lower steady state levels of ANP and constitutively released less ANP over time indicating that scaffold-imposed cell organization resulted in a shift in cell phenotype to a more mature state. We conclude that the physical organization of microfibers in ES-PU scaffolds impacts both multi-cellular architecture and cardiac cell phenotype in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.