ArticleBMC microbiology2008
Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 155 citations in OpenAlex.
- Gold Nanoparticle Paper Immunoassays for Sensing the Presence ofACS omega · 2023Article
- Development of the Droplet Digital PCR Method for the Detection and Quantification of Erwinia pyrifoliae.The plant pathology journal · 2023Article
- A Designed Vessel Using Dissolvable Polyvinyl Alcohol Membrane as Automatic Valve to Couple LAMP with CRISPR/Cas12a System for Visual Detection.Biosensors · 2023Article
- Improvements to the Rapid Detection of the Marine Pathogenic Bacterium,Microorganisms · 2022Article
- Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water.BMC microbiology · 2022Article
- An enzyme free fluorescence resonance transfer strategy based on hybrid chain reaction and triplex DNA for Vibrio parahaemolyticus.Scientific reports · 2020Article
- Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes.Sensors (Basel, Switzerland) · 2020Review
- Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.World journal of microbiology & biotechnology · 2020Article
- Clinical, Molecular and Serological Diagnosis of Canine Leishmaniosis: An Integrated Approach.Veterinary sciences · 2020Article
- Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits.Scientific reports · 2020Article
- CFD simulations for paper-based DNA amplification reaction (LAMP) of Mycobacterium tuberculosis-point-of-care diagnostic perspective.Medical & biological engineering & computing · 2020Article
- Article
- Loop-mediated isothermal amplification for visual detection of Vibrio parahaemolyticus using gold nanoparticles.Mikrochimica acta · 2017Article
- Development of a quadruplex loop-mediated isothermal amplification assay for field detection of four Vibrio species associated with fish disease.SpringerPlus · 2016Article
- Article
- Genome Wide Analysis for Rapid Identification of Vibrio Species.Indian journal of microbiology · 2015Article
- Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.The Journal of antimicrobial chemotherapy · 2015Review
- The pathogenesis, detection, and prevention of Vibrio parahaemolyticus.Frontiers in microbiology · 2015Article
- Loop-mediated isothermal amplification assays for screening of bacterial integrons.Biological research · 2014Article
- Rapid and simple detection of methicillin-resistance Staphylococcus aureus by orfX loop-mediated isothermal amplification assay.BMC biotechnology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio parahaemolyticus is a marine seafood-borne pathogen causing gastrointestinal disorders in humans. Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are known as major virulence determinants of V. parahaemolyticus. Most V. parahaemolyticus isolates from the environment do not produce TDH or TRH. Total V. parahaemolyticus has been used as an indicator for control of seafood contamination toward prevention of infection. Detection of total V. parahaemolyticus using conventional culture- and biochemical-based assays is time-consuming and laborious, requiring more than three days. Thus, we developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the sensitive and rapid detection of Vibrio parahaemolyticus.
resultsThe assay provided markedly more sensitive and rapid detection of V. parahaemolyticus strains than conventional biochemical and PCR assays. The assay correctly identified 143 V. parahaemolyticus strains, but did not detect 33 non-parahaemolyticus Vibrio and 56 non-Vibrio strains. Sensitivity of the LAMP assay for direct detection of V. parahaemolyticus in pure cultures and in spiked shrimp samples was 5.3 x 10(2) CFU per ml/g (2.0 CFU per reaction). The sensitivity of the LAMP assay was 10-fold more sensitive than that of the conventional PCR assay. The LAMP assay was markedly faster, requiring for amplification 13-22 min in a single colony on TCBS agar from each of 143 V. parahaemolyticus strains and less than 35 min in spiked shrimp samples. The LAMP assay for detection of V. parahaemolyticus required less than 40 min in a single colony on thiosulfate citrate bile salt sucrose (TCBS) agar and 60 min in spiked shrimp samples from the beginning of DNA extraction to final determination.
conclusionThe LAMP assay is a sensitive, rapid and simple tool for the detection of V. parahaemolyticus and will facilitate the surveillance for control of contamination of V. parahaemolyticus in seafood.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.