ArticlePlant methods2008
High-throughput Agrobacterium-mediated barley transformation.
Article in Plant methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
What it found
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Who cites it
85 citing papers in PubMed, 205 citations in OpenAlex.
- Composite Cucurbitaceae Plants as a Tool to Study Physiological and Molecular Mechanisms of Root Development.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Bt agave: why it is time to explore a new biotechnological frontier.Pest management science · 2026Review
- Controlling GRF4-GIF1 expression for efficient, genotype-independent transformation across wheat cultivars.The Plant journal : for cell and molecular biology · 2026Article
- Loss of E3 ligase HvST1 function substantially increases distal crossover frequency.The New phytologist · 2026Article
- Impact of wheat GRF4-GIF1 morphogenic regulators on transformation and genome editing efficiency in elite barley cultivars.Frontiers in plant science · 2026Article
- Transgenic barley over-expressingGM crops & food · 2025Article
- Nanoparticle-Mediated Nucleic Acid Delivery Systems in Plant Biotechnology: Recent Advances and Emerging Challenges.Plants (Basel, Switzerland) · 2025Review
- Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping.Nature plants · 2025Article
- Co-expression of growth regulator genes HvWUS and HvBBM2 enhances barley transformation efficiency.Plant physiology · 2025Article
- CRISPR/Cas9-Mediated Gene Knockout in Cereal Crops.Current protocols · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- MADS31 supports female germline development by repressing the post-fertilization programme in cereal ovules.Nature plants · 2025Article
- Hordeum I genome unlocks adaptive evolution and genetic potential for crop improvement.Nature plants · 2025Article
- Stress-Inducible Expression ofPlants (Basel, Switzerland) · 2024Article
- TFIIS is required for reproductive development and thermal adaptation in barley.Plant cell reports · 2024Article
- Breeding for an elite malting barley cultivar with acid soil tolerance.Communications biology · 2024Article
- Article
- Differential editing efficiencies in cereal crops: a comparative analysis of tRNA and ribozyme multiplexed guide delivery.Frontiers in plant science · 2024Article
- Barley TAPETAL DEVELOPMENT and FUNCTION1 (HvTDF1) gene reveals conserved and unique roles in controlling anther tapetum development in dicot and monocot plants.The New phytologist · 2023Article
- Rice transformation treatments leave specific epigenome changes beyond tissue culture.Plant physiology · 2023Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop in the world. It also provides a useful model for the study of wheat, which has a larger and more complex genome. Most existing barley transformation methodologies are either complex or have low (<10%) transformation efficiencies.
resultsA robust, simple and reproducible barley transformation protocol has been developed that yields average transformation efficiencies of 25%. This protocol is based on the infection of immature barley embryos with Agrobacterium strain AGL1, carrying vectors from the pBract series that contain the hpt gene (conferring hygromycin resistance) as a selectable marker. Results of large scale experiments utilising the luc (firefly luciferase) gene as a reporter are described. The method presented here has been used to produce hundreds of independent, transgenic plant lines and we show that a large proportion of these lines contain single copies of the luc gene.
conclusionThis protocol demonstrates significant improvements in both efficiency and ease of use over existing barley transformation methods. This opens up opportunities for the development of functional genomics resources in barley.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.