ArticleMolecular biotechnology2009
shRNA expression plasmids generated by a novel method efficiently induce gene-specific knockdown in a silkworm cell line.
Article in Molecular biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Doublesex and GATAβ4 synergistically regulate the sex-dimorphic expression of storage protein 1 in Bombyx mori.PLoS genetics · 2025Article
- CRISPR Disruption ofInsects · 2019Article
- Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus.Journal of virology · 2017Article
- Suppression of Bm-Caspase-1 Expression in BmN Cells Enhances Recombinant Protein Production in a Baculovirus Expression Vector System.Molecular biotechnology · 2016Article
- CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells.Scientific reports · 2015Article
- Enhancement of larval RNAi efficiency by over-expressing Argonaute2 in Bombyx mori.International journal of biological sciences · 2015Article
- Identification of a novel strong and ubiquitous promoter/enhancer in the silkworm Bombyx mori.G3 (Bethesda, Md.) · 2014Article
- Resistance of transgenic silkworm to BmNPV could be improved by silencing ie-1 and lef-1 genes.Gene therapy · 2014Article
- Serum-free culture of an embryonic cell line from Bombyx mori and reinforcement of susceptibility of a recombinant BmNPV by cooling.In vitro cellular & developmental biology. Animal · 2012Article
- A novel method to convert a DNA fragment inserted into a plasmid to an inverted repeat structure.Molecular biotechnology · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNAi knockdown by using shRNA expression plasmids is widely used to determine the function of individual genes in mammals. Here we developed a simple method to create an IR DNA in a U6 small nuclear RNA promoter-based parent vector using a single-stranded IR DNA with short hairpin structure and Bst DNA polymerase. Furthermore, we demonstrated that the shRNA expression plasmids constructed by our method effectively induced target-specific RNAi in the silkworm cell line. We also found that sequence preference in the silkworm cell line was much lower than in mammalian cells and shRNA-induced RNAi was influenced by the length of the stem region.
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18821064What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.