ArticleBiophysical journal2008
Spatial diffusivity and availability of intracellular calmodulin.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- Biomolecular condensation of cMLCK enables myosin motor phosphorylation in the heart.bioRxiv : the preprint server for biology · 2026Article
- Nernst-Planck-Gaussian finite element modelling of CaFrontiers in physiology · 2024Article
- On the role of calcium diffusion and its rapid buffering in intraflagellar signaling.European biophysics journal : EBJ · 2023Article
- Bound CaFrontiers in physiology · 2023Article
- A Modeling and Analysis Study Reveals That CaMKII in Synaptic Plasticity Is a Dominant Affecter in CaM Systems in a T286 Phosphorylation-Dependent Manner.Molecules (Basel, Switzerland) · 2022Article
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- A minimalist model to measure interactions between proteins and synaptic vesicles.Scientific reports · 2020Article
- Article
- Active dendrites regulate the spatiotemporal spread of signaling microdomains.PLoS computational biology · 2018Article
- Biophysical attributes that affect CaMKII activation deduced with a novel spatial stochastic simulation approach.PLoS computational biology · 2018Article
- Competitive tuning: Competition's role in setting the frequency-dependence of Ca2+-dependent proteins.PLoS computational biology · 2017Article
- Spatiotemporal Analysis of K-Ras Plasma Membrane Interactions Reveals Multiple High Order Homo-oligomeric Complexes.Journal of the American Chemical Society · 2017Article
- Signal Integration at Elongation Factor 2 Kinase: THE ROLES OF CALCIUM, CALMODULIN, AND SER-500 PHOSPHORYLATION.The Journal of biological chemistry · 2017Article
- Differential Regulation of PI(4,5)PFrontiers in molecular neuroscience · 2017Article
- Role of DARPP-32 and ARPP-21 in the Emergence of Temporal Constraints on Striatal Calcium and Dopamine Integration.PLoS computational biology · 2016Article
- Modulated CMOS camera for fluorescence lifetime microscopy.Microscopy research and technique · 2015Article
- Neurogranin regulates CaM dynamics at dendritic spines.Scientific reports · 2015Article
- Multiple spatial and kinetic subpopulations of CaMKII in spines and dendrites as resolved by single-molecule tracking PALM.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Pivoting between calmodulin lobes triggered by calcium in the Kv7.2/calmodulin complex.PloS one · 2014Article
- Towards the Physics of Calcium Signalling in Plants.Plants (Basel, Switzerland) · 2013Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Calmodulin (CaM) is the major pathway that transduces intracellular Ca2+ increases to the activation of a wide variety of downstream signaling enzymes. CaM and its target proteins form an integrated signaling network believed to be tuned spatially and temporally to control CaM's ability to appropriately pass signaling events downstream. Here, we report the spatial diffusivity and availability of CaM labeled with enhanced green fluorescent protein (eGFP)-CaM, at basal and elevated Ca2+,quantified by the novel fluorescent techniques of raster image scanning spectroscopy and number and brightness analysis. Our results show that in basal Ca2+ conditions cytoplasmic eGFP-CaM diffuses at a rate of 10 microm(2)/s, twofold slower than the noninteracting tracer, eGFP, indicating that a significant fraction of CaM is diffusing bound to other partners. The diffusion rate of eGFP-CaM is reduced to 7 microm(2)/s when a large (646 kDa) target protein Ca2+/CaM-dependent protein kinase II is coexpressed in the cells. In addition, the presence of Ca2+/calmodulin-dependent protein kinase II, which can bind up to 12 CaM molecules per holoenzyme, increases the stoichiometry of binding to an average of 3 CaMs per diffusive molecule. Elevating intracellular Ca2+ did not have a major impact on the diffusion of CaM complexes. These results present us with a model whereby CaM is spatially modulated by target proteins and support the hypothesis that CaM availability is a limiting factor in the network of CaM-signaling enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.