ArticleEukaryotic cell2008
UGE1 and UGE2 regulate the UDP-glucose/UDP-galactose equilibrium in Cryptococcus neoformans.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Intrinsic and extrinsic factors affecting the evolution of virulence in the HIV-associated opportunistic human fungal pathogenVirulence · 2025Article
- Article
- Alternative TSS use is widespread in Cryptococcus fungi in response to environmental cues and regulated genome-wide by the transcription factor Tur1.PLoS biology · 2024Article
- Identification of a putative α-galactoside β-(1 → 3)-galactosyltransferase involved in the biosynthesis of galactomannan side chain of glucuronoxylomannogalactan inFrontiers in microbiology · 2024Article
- Article
- Article
- Application of an optimized annotation pipeline to the Cryptococcus deuterogattii genome reveals dynamic primary metabolic gene clusters and genomic impact of RNAi loss.G3 (Bethesda, Md.) · 2021Article
- Quantitative global studies reveal differential translational control by start codon context across the fungal kingdom.Nucleic acids research · 2020Article
- Cryptococcus neoformans resists to drastic conditions by switching to viable but non-culturable cell phenotype.PLoS pathogens · 2019Article
- The birth, evolution and death of metabolic gene clusters in fungi.Nature reviews. Microbiology · 2018Review
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter.Glycobiology · 2017Article
- Intron retention-dependent gene regulation in Cryptococcus neoformans.Scientific reports · 2016Article
- Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans.The Journal of biological chemistry · 2016Article
- Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis.Cold Spring Harbor perspectives in medicine · 2014Review
- Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.PLoS genetics · 2014Article
- Elucidation of substrate specificity in Aspergillus nidulans UDP-galactose-4-epimerase.PloS one · 2013Article
- A defect in ATP-citrate lyase links acetyl-CoA production, virulence factor elaboration and virulence in Cryptococcus neoformans.Molecular microbiology · 2012Article
- Aimless mutants of Cryptococcus neoformans: failure to disseminate.Fungal biology reviews · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of the basidiomycete pathogenic yeast Cryptococcus neoformans carries two UDP-glucose epimerase genes (UGE1 and UGE2). UGE2 maps within a galactose cluster composed of a galactokinase homologue gene and a galactose-1-phosphate uridylyltransferase. This clustered organization of the GAL genes is similar to that in most of the hemiascomycete yeast genomes and in Schizosaccharomyces pombe but is otherwise not generally conserved in the fungal kingdom. UGE1 has been identified as necessary for galactoxylomannan biosynthesis and virulence. Here, we show that UGE2 is necessary for C. neoformans cells to utilize galactose as a carbon source at 30 degrees C but is not required for virulence. In contrast, deletion of UGE1 does not affect cell growth on galactose at this temperature. At 37 degrees C, a uge2Delta mutant grows on galactose in a UGE1-dependent manner. This compensation by UGE1 of UGE2 mutation for growth on galactose at 37 degrees C was not associated with upregulation of UGE1 transcription or with an increase of the affinity of the enzyme for UDP-galactose at this temperature. We studied the subcellular localization of the two enzymes. Whereas at 30 degrees C, Uge1p is at least partially associated with intracellular vesicles and Uge2p is on the plasma membrane, in cells growing on galactose at 37 degrees C, Uge1p colocalizes with Uge2p to the plasma membrane, suggesting that its activity is regulated through subcellular localization.
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