ArticleJournal of biomolecular NMR2008
Detection of protein-ligand interactions by NMR using reductive methylation of lysine residues.
Article in Journal of biomolecular NMR, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Novel peptide nanoparticle-biased antagonist of CCR3 blocks eosinophil recruitment and airway hyperresponsiveness.The Journal of allergy and clinical immunology · 2019Article
- Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy.Methods in enzymology · 2019Article
- Current NMR Techniques for Structure-Based Drug Discovery.Molecules (Basel, Switzerland) · 2018Review
- Capturing the photo-signaling state of a photoreceptor in a steady-state fashion by binding a transition metal complex.Protein science : a publication of the Protein Society · 2017Article
- Molecular Interactions of Lipopolysaccharide with an Outer Membrane Protein from Pseudomonas aeruginosa Probed by Solution NMR.Biochemistry · 2016Article
- Evaluation of colorimetric assays for analyzing reductively methylated proteins: Biases and mechanistic insights.Analytical biochemistry · 2015Article
- 13C NMR detects conformational change in the 100-kD membrane transporter ClC-ec1.Journal of biomolecular NMR · 2015Article
- A selective NMR probe to monitor the conformational transition from inactive to active kinase.ACS chemical biology · 2015Article
- Application of reductive ¹³C-methylation of lysines to enhance the sensitivity of conventional NMR methods.Molecules (Basel, Switzerland) · 2013Article
- Utilization of lysine ¹³C-methylation NMR for protein-protein interaction studies.Journal of biomolecular NMR · 2013Article
- Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins.Advances in experimental medicine and biology · 2012Review
- Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor.Nature · 2010Article
- Differences in lysine pKa values may be used to improve NMR signal dispersion in reductively methylated proteins.Journal of biomolecular NMR · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We show that reductive methylation of proteins can be used for highly sensitive NMR identification of conformational changes induced by metal- and small molecule binding, as well as protein-protein interactions. Reductive methylation of proteins introduces two (13)C-methyl groups on each lysine in the protein of interest. This method works well even when the lysines are not actively involved in the interaction, due to changes in the microenvironments of lysine residues. Most lysine residues are located on the protein exterior, and the exposed (13)C-methyl groups may exhibit rapid localized motions. These motions could be faster than the tumbling rate of the molecule as a whole. Thus, this technique has great potential in the study of large molecular weight systems which are currently beyond the scope of conventional NMR methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.