ArticleProceedings of the National Academy of Sciences of the United States of America2008
Tetrapyrrole profiling in Arabidopsis seedlings reveals that retrograde plastid nuclear signaling is not due to Mg-protoporphyrin IX accumulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
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Who cites it
118 citing papers in PubMed, 251 citations in OpenAlex.
- Introduction of regioselective bacterial heme oxygenases into Arabidopsis hy1-1 supports the retrograde heme signaling hypothesis.Plant physiology · 2026Article
- Revealing Missing Links in the Downsizing of the Photosystem II Antenna in Higher Plants Under Stress Conditions.Antioxidants (Basel, Switzerland) · 2025Article
- Plastids in a Pinch: Coordinating Stress and Developmental Responses Through Retrograde Signalling.Plant, cell & environment · 2025Review
- Arabidopsis transcription factor ANAC102 predominantly expresses a nuclear protein and acts as a negative regulator of methyl viologen-induced oxidative stress responses.Journal of experimental botany · 2024Article
- Orchestration of Photosynthesis-Associated Gene Expression and Galactolipid Biosynthesis during Chloroplast Differentiation in Plants.Plant & cell physiology · 2024Article
- Physiological, Cytological, and Transcriptomic Analysis of Magnesium Protoporphyrin IX Methyltransferase Mutant Reveal Complex Genetic Regulatory Network Linking Chlorophyll Synthesis and Chloroplast Development in Rice.Plants (Basel, Switzerland) · 2023Article
- Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell.Communications biology · 2023Article
- Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS.Plant methods · 2023Article
- Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.Plant communications · 2023Review
- Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants.Plant communications · 2023Article
- Advances in Iron Retrograde Signaling Mechanisms and Uptake Regulation in Photosynthetic Organisms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Retrograde and anterograde signaling in the crosstalk between chloroplast and nucleus.Frontiers in plant science · 2022Review
- Chimeric Mouse With Humanized Liver Is an Appropriate Animal Model to Investigate Mode of Action for Porphyria-Mediated Hepatocytotoxicity.Toxicologic pathology · 2021Article
- Alleviation of norflurazon-induced photobleaching by overexpression of Fe-chelatase in transgenic rice.Journal of pesticide science · 2021Article
- Chlorophyllides: Preparation, Purification, and Application.Biomolecules · 2021Review
- GUN control in retrograde signaling: How GENOMES UNCOUPLED proteins adjust nuclear gene expression to plastid biogenesis.The Plant cell · 2021Review
- Article
- The Role of Tetrapyrrole- and GUN1-Dependent Signaling on Chloroplast Biogenesis.Plants (Basel, Switzerland) · 2021Review
- A Holistic Approach to Study Photosynthetic Acclimation Responses of Plants to Fluctuating Light.Frontiers in plant science · 2021Review
- Characterization and Fine Mapping of a Yellow-Virescent Gene Regulating Chlorophyll Biosynthesis and Early Stage Chloroplast Development inG3 (Bethesda, Md.) · 2020Article
58 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
Funding
Abstract
Chloroplast biogenesis involves careful coordination of both plastid and nuclear gene expression, which is achieved in part by retrograde signaling from the chloroplast to the nucleus. This can be demonstrated by the fact that the herbicide, Norflurazon (NF), which causes bleaching of chloroplasts, prevents the light induction of photosynthesis-related genes in the nucleus. It has been proposed that the tetrapyrrole pathway intermediate Mg-protoporphyrin IX acts as the signaling molecule in this pathway and accumulates in the chloroplasts and cytosol of the cell after NF treatment. Here we present data that demonstrate that this model is too simplistic. We have developed a sensitive liquid chromatography-mass spectrometry (LC/MS) method to measure tetrapyrrole intermediates and have shown that no Mg-protoporphyrin IX, nor indeed any other chlorophyll-biosynthesis intermediate, can be detected in NF-treated plants under conditions in which nuclear gene expression is repressed. Conversely when endogenous Mg-protoporphyrin IX levels are artificially increased by supplementation with the tetrapyrrole precursor, 5-aminolevulinic acid, the expression of nuclear-encoded photosynthetic genes is induced, not repressed. We also demonstrate that NF-treatment leads to a strong down-regulation of tetrapyrrole biosynthesis genes, consistent with the absence of an accumulation of tetrapyrrole intermediates. Finally, there is no correlation between nuclear-gene expression and any of the chlorophyll biosynthetic intermediates over a range of growth conditions and treatments. Instead, it is possible that a perturbation of tetrapyrrole synthesis may lead to localized ROS production or an altered redox state of the plastid, which could mediate retrograde signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.