ArticleProceedings of the National Academy of Sciences of the United States of America2008
S4-based voltage sensors have three major conformations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 159 papers.
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159 citing papers in PubMed, 211 citations in OpenAlex.
- Molecular Determinants of Voltage-Dependent Gating in Kv11.1 Channels.Advances in experimental medicine and biology · 2026Review
- Closed state structure of the pore revealed by uncoupled Shaker KNature communications · 2025Article
- Voltage sensor conformations induced by LQTS-associated mutations in hERG potassium channels.Nature communications · 2025Article
- Energy landscape of a Kv channel revealed by temperature steps while perturbing its electromechanical coupling.Nature communications · 2025Article
- Inactivation of CaV1 and CaV2 channels.The Journal of general physiology · 2025Review
- Voltage clamp fluorometry inBio-protocol · 2025Article
- The significance of electrical signals in maturing spermatozoa for phosphoinositide regulation through voltage-sensing phosphatase.Nature communications · 2024Article
- Different fluorescent labels report distinct components of spHCN channel voltage sensor movement.The Journal of general physiology · 2024Article
- Hydrophobic residues in S1 modulate enzymatic function and voltage sensing in voltage-sensing phosphatase.The Journal of general physiology · 2024Article
- Effect of a sensing charge mutation on the deactivation of KV7.2 channels.The Journal of general physiology · 2024Article
- Dynamics of activation in the voltage-sensing domain of Ciona intestinalis phosphatase Ci-VSP.Nature communications · 2024Article
- Non-canonical helical transitions and conformational switching are associated with characteristic flexibility and disorder indices in TRP and Kv channels.Channels (Austin, Tex.) · 2023Article
- Fifty years of gating currents and channel gating.The Journal of general physiology · 2023Review
- Molecular rearrangements in S6 during slow inactivation in Shaker-IR potassium channels.The Journal of general physiology · 2023Article
- Role of K364 next to the active site cysteine in voltage-dependent phosphatase activity of Ci-VSP.Biophysical journal · 2023Article
- Optical measurement of gating pore currents in hypokalemic periodic paralysis model cells.Disease models & mechanisms · 2023Article
- Charge-voltage curves of Shaker potassium channel are not hysteretic at steady state.The Journal of general physiology · 2023Article
- Multiple mechanisms underlie reduced potassium conductance in the p.T1019PfsX38 variant of hERG.Physiological reports · 2022Article
- A trafficking motif alters GEVI activity implicating persistent protein interactions at the membrane.Biophysical reports · 2022Article
- Hysteretic hERG channel gating current recorded at physiological temperature.Scientific reports · 2022Article
99 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
Funding
Abstract
Voltage sensors containing the charged S4 membrane segment display a gating charge vs. voltage (Q-V) curve that depends on the initial voltage. The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows the same phenomenon and the Q-V recorded with a depolarized initial voltage is more stable by at least 3RT. The leftward shift of the Q-V curve under prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed fluorescence measurements. The fluorescence shows two components: one that traces the time course of the charge movement between the resting and active states and a slower component that traces the transition between the active state and a more stable state we call the relaxed state. Temperature dependence shows a large negative enthalpic change when going from the active to the relaxed state that is almost compensated by a large negative entropic change. The Q-V curve midpoint measured for pulses that move the sensor between the resting and active states, but not long enough to evolve into the relaxed states, show a periodicity of 120 degrees, indicating a 3(10) secondary structure of the S4 segment when determined under histidine scanning. We hypothesize that the S4 segment moves as a 3(10) helix between the resting and active states and that it converts to an alpha-helix when evolving into the relaxed state, which is most likely to be the state captured in the crystal structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.