ArticleThe Journal of biological chemistry2008
Binding of the unorthodox transcription activator, Crl, to the components of the transcription machinery.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Bimodality in E. coli gene expression: Sources and robustness to genome-wide stresses.PLoS computational biology · 2025Article
- Article
- Structural basis for transcription activation by Crl through tethering of σProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The pneumococcal σThe Journal of biological chemistry · 2019Article
- Regulation of Global Transcription inG3 (Bethesda, Md.) · 2018Article
- Binding interface between the Salmonella σ(S)/RpoS subunit of RNA polymerase and Crl: hints from bacterial species lacking crl.Scientific reports · 2015Article
- Repressor activity of the RpoS/σS-dependent RNA polymerase requires DNA binding.Nucleic acids research · 2015Article
- Structure of the RNA polymerase assembly factor Crl and identification of its interaction surface with sigma S.Journal of bacteriology · 2014Article
- Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation.Journal of bacteriology · 2014Article
- Transcriptional occlusion caused by overlapping promoters.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Key features of σS required for specific recognition by Crl, a transcription factor promoting assembly of RNA polymerase holoenzyme.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase.Nucleic acids research · 2013Article
- Mycobacterium tuberculosis RNA polymerase-binding protein A (RbpA) and its interactions with sigma factors.The Journal of biological chemistry · 2013Article
- Crl binds to domain 2 of σ(S) and confers a competitive advantage on a natural rpoS mutant of Salmonella enterica serovar Typhi.Journal of bacteriology · 2010Article
- Identification of conserved amino acid residues of the Salmonella sigmaS chaperone Crl involved in Crl-sigmaS interactions.Journal of bacteriology · 2010Article
- RNA polymerase: a nexus of gene regulation.Methods (San Diego, Calif.) · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The small regulatory protein Crl binds to sigmaS, the RNA polymerase stationary phase sigma factor. Crl facilitates the formation of the sigmaS-associated holoenzyme (EsigmaS) and thereby activates sigmaS-dependent genes. Using a real time surface plasmon resonance biosensor, we characterized in greater detail the specificity and mode of action of Crl. Crl specifically forms a 1:1 complex with sigmaS, which results in an increase of the association rate of sigmaS to core RNA polymerase without any effect on the dissociation rate of EsigmaS. Crl is also able to associate with preformed EsigmaS with a higher affinity than with sigmaS alone. Furthermore, even at saturating sigmaS concentrations, Crl significantly increases EsigmaS association with the katN promoter and the productive isomerization of the EsigmaS-katN complex, supporting a direct role of Crl in transcription initiation. Finally, we show that Crl does not bind to sigma70 itself but is able at high concentrations to form a weak and transient 1:1 complex with both core RNA polymerase and the sigma70-associated holoenzyme, leaving open the possibility that Crl might also exert a side regulatory role in the transcriptional activity of additional non-sigmaS holoenzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.